FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24178
Total Bases3.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA757831.34254280751096No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT439318.16941020762677No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA430217.793034990487218No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT255910.58400198527587No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC13295.496732566796261No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2070.8561502192075441No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGTA1670.6907105633220283Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1640.6783025891306146No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT1450.5997187525849946No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT1110.4590950450823062No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAA1060.4384150880966168Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT960.39705517412523783No Hit
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGTA930.3846471999338241Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGGT910.37637521713954836Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT830.3432872859624452No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT730.3019273719910663No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC690.2853834064025147No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT560.23161551823972204No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT440.18198362147406735No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG420.17371163867979156No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT340.14062370750268838No Hit
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGAA320.1323517247084126Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGGTGGAGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT310.12821573331127473No Hit
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGGT300.12407974191413682Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT270.11167176772272315No Hit
AAGGTGGATGGGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT260.10753577632558525No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)