FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12905
Total Bases1.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT500238.76017047655947No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC201715.629600929872142No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA169313.118946144905074No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA167112.948469585432004No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT2902.247191011235955No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2331.8055017435102672No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2161.6737698566447115No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG540.41844246416117786No Hit
TGCCATCTTGCCACATCTGACCCCATGATATGTATTGTTAACTGACAGAA360.27896164277411856No Hit
TGCCATCTTGCCACATCTTCTGCTCATCGATCCTGGGGTTTGGGGTTTAT330.2557148392096087No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG300.2324680356450988No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT210.16272762495156914No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT200.15497869043006587No Hit
TGCCATCTTGCCACATCTGTTTTAAGATGGATTCAGCATGATCACATGTA200.15497869043006587No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT190.14722975590856258No Hit
TGCCATCTTGCCACATCTTAGCAAGGGTATCCGCCAACTTGAGGAGACCA190.14722975590856258No Hit
TGCCATCTTGCCACATCTTACCCCATGATATGTATTGTTAACTGACAGAA170.13173188686555598No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT170.13173188686555598No Hit
GAGGACATCTTATGGTGCATGGGGGCAAACTGAGGTATTGAGGAGACCAA160.12398295234405268No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT160.12398295234405268No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG150.1162340178225494No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT150.1162340178225494No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGCTTCCCAGT150.1162340178225494No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT140.1084850833010461No Hit
TGCCATCTTGCCACATCTTAGGGTATCCGCCAACTTGAGGAGACCAAGTC140.1084850833010461No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGC140.1084850833010461No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT130.10073614877954282No Hit
TGCCATTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT130.10073614877954282No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT130.10073614877954282No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)