FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371
Total Bases53.7 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG8021.563342318059302No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG4913.20754716981132No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT379.973045822102426No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC297.816711590296496No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC102.6954177897574128No Hit
AAGGTGGATGGTGATGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT71.8867924528301887No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT71.8867924528301887No Hit
GAGGACATCTTATGGTGCACTGAAGCAGTGGTATCAACGCAGAGTACATG61.6172506738544474Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCCTCTCTGCCTTTTCTCAAGCAGTGGTATCAACGCAGAGTACATGGG61.6172506738544474Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGGTGGATGGTGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTC61.6172506738544474No Hit
TGCCATCTTGCCACATCTTAGTATCCGCCAACACAGAAGGGTATCCGCCA51.3477088948787064No Hit
AAGGTGGATGGTGATGTGGTATCAACGCAGAGTACATGGGTATCCGCCAA51.3477088948787064No Hit
GAGGACATCTTATGGTGCACGGGGGCAAACTGAGGTATTGAGGAGACCAA41.078167115902965No Hit
AAGGTGGATGGTGATGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA41.078167115902965No Hit
GAGGACATCTTATGGTGCACTGGGGCAAACTGAGGTATTGAGGAGACCAA41.078167115902965No Hit
TGCCATCTTGCCACATCGCTCTTCCGATCTTCAACAGAATGGAAGGGTAT41.078167115902965No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG41.078167115902965No Hit
TGCCATCTTGCCACATCTGGAAGGGTATCCGCCAACTTGAGGAGACCAAG41.078167115902965No Hit
AAGGTGGATGGTGATGTCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTC30.8086253369272237No Hit
TGCCATCTTGCCACATCTCTCTTCCGATCTTCAACAGAATGGAAGGGTAT30.8086253369272237No Hit
AAGGTGGATGGTGATGTGGTCAAGCAGTGGTATCAACGCAGAGTACATGG30.8086253369272237Clontech Universal Primer Mix Long (96% over 27bp)
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG30.8086253369272237No Hit
GAGGACATCTTATGGTGCATGGGGGCAAACTGAGGTATTGAGGAGACCAA20.5390835579514826No Hit
TGCCATCTTGCCACATCTCTCTTCCGATCTTCAACAGAACGGAAGGGTAT20.5390835579514826No Hit
TGCCATCTTGCCACATCTTTCTTCCGATCTTCAACAGAATGGAAGGGTAT20.5390835579514826No Hit
AAGGTGGATGCCGCTGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTACC20.5390835579514826No Hit
TGCCATCTTGCCACATCGCTCTTCCGATCTTCAACAGAACGGAAGGGTAT20.5390835579514826No Hit
GAGGACATCTTATGGTGCACTGGGGGCAAACTGAGGTATTGAGGAGACCA20.5390835579514826No Hit
GAGGACATCTTATGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC20.5390835579514826No Hit
GAGGACATCTTATGGTGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCT20.5390835579514826No Hit
AAGGTGGATGGTGATGTGGTATCAACGCAGAGTACATGGGGTGGTATCAA20.5390835579514826No Hit
GAGGACATCTTATGGTGCACTGGGGCAAACTAGGTATTGAGGAGACCAAG10.2695417789757413No Hit
AAGGTGGATGGTGAAGTGGTATCAACGCAGAGTACATGGGTATCCGCCAA10.2695417789757413Clontech SMARTer II A Oligonucleotide (95% over 23bp)
TGCCATCTTGCCACATCTTGGTATCCGCCAACACAGAAGGGTATCCGCCC10.2695417789757413No Hit
AAGGTGGATGGTGATGTGGTATCAACGCAGAGTTGCTGTCATTGGTCCGA10.2695417789757413No Hit
TGCCATCTTGCCACATCTTGGTATCCGCCAACACAGAAGGGTATCCGCCA10.2695417789757413No Hit
TGCCATCTTGCCACATCTTAGGGAGACCAAGCATGGGGGCAAACTGAGGT10.2695417789757413No Hit
TGCCATCTTGCCAACTTGAGGAGACCAAGTCTCTGCTACCGTAATCGCAT10.2695417789757413No Hit
GAGGACATCTTATGGGGGCAAACTGGGGTATTGAGGAGACCAAGTCTCTG10.2695417789757413No Hit
GAGGACATCTTATGGTGGCAAATGAGGTATTGAGGAGACCAAGTCTCTGC10.2695417789757413No Hit
TGCCCTCTCTGCCTTTTCTTAAGCAGTGGTATCAACGCAGAGTACATGGG10.2695417789757413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACCTCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG10.2695417789757413No Hit
GAGGACATCTTATTGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG10.2695417789757413No Hit
TGCCATCTTGCCACATCTTGTGCCATCTTGCCACAGGAAGGGTATCCGCC10.2695417789757413No Hit
GAGGACATCTTATGGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCT10.2695417789757413No Hit
TGCCATCTTGCCACATCTCTCTTCCGATCTTCAACAGAACAGAAGGGTAT10.2695417789757413No Hit
AAGGTGGATGGGAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10.2695417789757413Clontech SMARTer II A Oligonucleotide (95% over 23bp)
AAGGTGGATGGTGATGTGGTCAAGCAGTGGTATCAATGCAGAGTACATGG10.2695417789757413Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA10.2695417789757413No Hit
GAGGACATCTTATGGGGGCAAACGAGGTATTGAGGAGACCAAGTCTCTGC10.2695417789757413No Hit
TGCCATCTTGCCACATCTTAGAAGGGTATCCGCCAACTTGAGGAGACCAA10.2695417789757413No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGGCTCTGC10.2695417789757413No Hit
TGCCATCTTGCCACATCTTAGGTATCCGCCAGCACAGAAGGGTATCCGCC10.2695417789757413No Hit
TGCCATCTTGCCACATCTTGGTATCCGCCAACACAGGGTATCCGCCAACA10.2695417789757413No Hit
AAGGTGGATGGTGATGTGGTCAGATGGCAAACTGAGGTAGCGACACTTGA10.2695417789757413No Hit
AAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG10.2695417789757413No Hit
TGCCATTTGCCACATCTGTGAAGGGTATCCGCCAACTTGAGGAGACCAAG10.2695417789757413No Hit
AAGGTGGAGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTA10.2695417789757413No Hit
GCCATCTTGCCACATCTGGGTATCCGCCAACTTGAGGAGACCAAGTCTCT10.2695417789757413No Hit
AAGGTGGATGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTA10.2695417789757413No Hit
TGCCATCTTGCCACATCTTAGAAAGCAGTGGTATCAACGCAGAGTACATG10.2695417789757413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGAGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG10.2695417789757413No Hit
TGCCATCTTGCCACATCTTGGTATCCGCCAACTTGAGGAGACCAAGTCTC10.2695417789757413No Hit
AAGGTGGATGGTGATGTGGTAAAGCAGTGGTATCAACGCAGAGTACATGG10.2695417789757413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGGGGCAAACTGAGGCATTGAGGAGACCAAGTCTCTG10.2695417789757413No Hit
GAGGACCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGCT10.2695417789757413No Hit
TGCCATCTTGCCACATCTGCCAACACAGAAGGGTATCCGCCAACACAGGG10.2695417789757413No Hit
TGCCATCTTGCCACATCTGGGGTATCCGCCAACTTGAGGAGACCAAGTCT10.2695417789757413No Hit
TGCCATCTTGCCACATCTTAGGGGGGAAGGGTATCCGCCAACTTGAGGAG10.2695417789757413No Hit
TGCCATCTTGCCACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCA10.2695417789757413No Hit
TGCCATCTTGCCACATCTTAGTATCCGCCAACGCAGTGGTATCAACGCAG10.2695417789757413No Hit
GAGGACATCTTATGGTGCACTGAGGTATTGAGGAGACCAAGTCTCTGCTA10.2695417789757413No Hit
AAGGTGGATGGTGATCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT10.2695417789757413No Hit
AAGGTGGATGCAGCTGGTCTACTACTTGAGGAGACCAAGTCTCTGCAACA10.2695417789757413No Hit
AAGGTGGTGGTGATGTGGTATCAACGCAGAGTACATGGGTATCCGCCAAC10.2695417789757413No Hit
GAGGACATCTTATGGTGGAAAACTGAGGTATTGAGGAGACCAAGTCTCTG10.2695417789757413No Hit
AAGGTGGATGGTGATGGGTCTATTTCGTTGAGGAGACCAAGTCTCTGCTA10.2695417789757413No Hit
AAGGTGGATGGTGATGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTACC10.2695417789757413No Hit
TGCCCTCTCTGCCTTTTCTAAGCAGTGGTATCAACGCAGAGTACATGGGA10.2695417789757413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCCTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG10.2695417789757413No Hit
TGCCATCTTGCCACATCTTGGGGGTATCCGCCAACACAGGAAGGGTATCC10.2695417789757413No Hit
TGCCATCTTGCCACATCTGGTATCCGCCAACACAGCAAGGGTATCCGCCA10.2695417789757413No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACACAGGGGTATCCGCCAA10.2695417789757413No Hit
GAGGACATCTATGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGCT10.2695417789757413No Hit
AAGGTGGATGGTGCTGGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGC10.2695417789757413No Hit
TGCCATCTTGCCACATCTAGGGTATCCGCCAACACAGAAGGGTATCCGCC10.2695417789757413No Hit
TGCCATCTTGCCACATCTTAGGGGGGGGAAGGGTATCCGCCAACTTGAGG10.2695417789757413No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACAAAGTCTCTG10.2695417789757413No Hit
AAGGTGGATGGTGAGTGGTATCAACGCAGAGTACATGGGTATCCGCCAAC10.2695417789757413Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AAGGTGGATGGTGATGTGGTATCAACGCAGAGTACATGTAAGCAGTGGTA10.2695417789757413No Hit
TGCCATCTTGCCACATCTAGAGGGTATCCGCCAACACAGAAGGGTATCCG10.2695417789757413No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACAAGTCTC10.2695417789757413No Hit
TGCCATCTTGCCACATCTTCACCTGACAATGTCTTCCGCCCGGATCGGCC10.2695417789757413No Hit
GAGACATCTTATGGTGCACTGAAATGGAAAGTGTATTTGGAATCCCAGTC10.2695417789757413No Hit
TGCCATCTTGCCACATCTAGGGTATCCGCCAACTTGAGGAGACCAAGTCT10.2695417789757413No Hit
TGCCATCTTGCCACATCTTAGGGGTATCCGCCAACACAGAAGGGTATCCG10.2695417789757413No Hit
AAGGTGATGGTGATGTGGTATCAACGCAGAGTACATGGAAGCAGTCGTAT10.2695417789757413No Hit
TGCCATCTTGCCACATCTGTGGTATCAACGCAGAGTACATGGGTATCCGC10.2695417789757413Clontech SMARTer II A Oligonucleotide (95% over 22bp)
AAGGTGGATGGTGATGTGGGGGGGGAGTGGTATCAACGCAGAGTACATGG10.2695417789757413Clontech SMARTer II A Oligonucleotide (95% over 23bp)
AAGGTGGATGGTGATGCCGCCGGTCTACTTCGTTGAGGAGACCAAGTCTC10.2695417789757413No Hit
TACCATCTTGCCACATCTCTCTTCCGATCTTCAACAGAATGGAAGGGTAT10.2695417789757413No Hit

[FAIL]Adapter Content

Adapter graph