FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11980
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length134-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA170014.190317195325541No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT8847.378964941569283No Hit
TGCCATCTTGCCACATCTACGCAGAGTACATGGGAAGCAGTGGTATCAAC6495.417362270450751No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2772.3121869782971616No Hit
TGCCATCTTGCCACATCTGGAAGCAGTGGTATCAACGCAGAGTACATGGG2562.1368948247078463Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTGAAGCAGTGGTATCAACGCAGAGTACATGGG2351.961602671118531Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1981.6527545909849748No Hit
TGCCATCTTGCCACATCTACTCTGCGTTGATACCACTGCTTCCCATGTAC1941.6193656093489148Clontech Universal Primer Mix Long (96% over 26bp)
TGCCATCTTGCCACATCTTCCCCCGAAGCAGTGGTATCAACGCAGAGTAC1811.5108514190317197Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT1471.2270450751252087No Hit
TGCCATCTTGCCACATCTTCCTGTGCCATCTTGCCACATCTGAAGCAGTG1471.2270450751252087No Hit
TGCCATCTTGCCACATCTTCGCCCAAGGACGTCCCATGTACTCTGCGTTG1180.9849749582637729No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC900.7512520868113522No Hit
TGCCATCTTGCCACATCTTAGGGCAAAGACCAGCATTTTCCGGAAGCAGT820.6844741235392321No Hit
TGCCATCTTGCCACATCTTCGCAGAGTACATGGGAAGCAGTGGTATCAAC720.6010016694490818No Hit
TGCCATCTTGCCACATCGAAGCAGTGGTATCAACGCAGAGTACATGGGAA680.5676126878130217Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTACGTCCTTGGGCGAAAAGGGTTGGACATCATC650.5425709515859767No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT650.5425709515859767No Hit
TGCCATCTTGCCACATCTACGCAGAGTACATGGAAAGCAGTGGTATCAAC620.5175292153589316No Hit
TGCCATCTTGCCACATCTCCCTGTGCCATCTTGCCACATCTGAAGCAGTG580.48414023372287146No Hit
TGCCATCTTGCCACATCTGAAAGCAGTGGTATCAACGCAGAGTACATGGG510.42570951585976624Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTCAGAAGGGTATCCGCCAACTTGAGGAGACCA490.4090150250417362No Hit
TGCCATCTTGCCACATCTTGTGTTGGCGGGGGCAAACTGAGGTATTGAGG480.40066777963272115No Hit
TGCCATCTTGCCACATCTCCCGCGCCCCGCCCCCATGTACTCTGCGTTGA470.39232053422370616No Hit
TGCCATCTTGCCACATCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC400.333889816360601No Hit
TGCCATCTTGCCACATCTTAGACCCACTCAGTGCGGGTATGGAAAATGCT400.333889816360601No Hit
TGCCATCTTGCCACATCGAAGCAGTGGTATCAACGCAGAGTACATGGGTA390.325542570951586Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTGATCTGAGGTCGCGCCCATGTACTCTGCGTTG380.31719532554257096No Hit
TGCCATCTTGCCACATCTTATCTGAGGTCGCGAAGGGTATCCGCCAACTT340.2838063439065108No Hit
TGCCATCTTGCCACATCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT320.2671118530884808No Hit
TGCCATCTTGCCACATCTGAAGCAGTGGTATCAACGCAGAGTACATGGGA320.2671118530884808Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGGTGGATGGTGATGTGGTGGGGGAGGAGGAAGGCGGGTCCGGAAGGGG310.2587646076794658No Hit
TGCCATCTTGCCACATCTCTGTGTTGGCGGATACACTGCTTCCCATGTAC270.22537562604340566No Hit
TGCCATCTTGCCACATCTTAGGGCGAAAAGGGTTGGACATCATCCGGGAA260.21702838063439064No Hit
TGCCATCTTGCCACATCTTGTCCTTGGGCGAAAAGGGTTGGACATCATCC250.20868113522537562No Hit
TGCCATCTTGCCACATCTACGTCCTTGGGCGAAAAGGGTTGGACATCATG250.20868113522537562No Hit
TGCCATCTTGCCACATCTTAGGGCGAAAAGGGTTGGACATCATCCCGGAA240.20033388981636058No Hit
AAGGTGGATGGTGATGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT240.20033388981636058No Hit
TGCCATCTTGCCACATCTTCAAAGTTCTTTTCAACTTTCCCTTCCCATGT240.20033388981636058No Hit
TGCCATCTTGCCACATCTCTTCAAAGTTCTTTTCAACTTTCCCTTCCCAT240.20033388981636058No Hit
TGCCATCTTGCCACATCTTGGGGCAAAGACCAGCATTTTCCGGAAGCAGT230.19198664440734559No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT230.19198664440734559No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC230.19198664440734559No Hit
TGCCATCTTGCCACATCTACTCTGCGTTGATACCACTGCTTCCCATGGGG220.18363939899833057Clontech Universal Primer Mix Long (96% over 26bp)
TGCCATCTTGCCACATCTGTGCCATCTTGCCCCCCGGAAGCAGTGGTATC220.18363939899833057No Hit
TGCCATCTTGCCACATCTCTGCGTTGATACCACTGCTTCCCATGTACTCT210.17529215358931552Clontech Universal Primer Mix Long (95% over 23bp)
AAGGTGGATGGTGATGTGTATAAGAGACAGGACTACAAGGGTATCCGCCA210.17529215358931552No Hit
TGCCATCTTGCCACATCTTCCGCGCCCCGCCCCCATGTACTCTGCGTTGA190.15859766277128548No Hit
TGCCATCTTGCCACATCTAGAAAAGGTGTTGGTTGATATAGACAGCAGGA190.15859766277128548No Hit
TGCCATCTTGCCACATCTTCGCCCAAGGGTATCCGCCAACTTGAGGAGAC180.15025041736227046No Hit
TGCCATCTTGCCACATCTTCCCCCAAAGCAGTGGTATCAACGCAGAGTAC180.15025041736227046Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTCGTCCTTGGGCGAAAAGGGTTGGACATCATCC170.1419031719532554No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT170.1419031719532554No Hit
TGCCATCTTGCCACATCTTAGGCGAAAAGGGTTGGACATCATCCCGGAAG170.1419031719532554No Hit
TGCCATCTTGCCACATCTTAGAGCAAAGACCAGCATTTTCCGGAAGCAGT160.1335559265442404No Hit
TGCCATCTTGCCACATCTGGAAGCAGTGGTATCAACGCAGAGTACATGGA160.1335559265442404Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTCCGGAAGGGGAAGGGTATCCGCCAACTTGAGG160.1335559265442404No Hit
TGCCATCTTGCCACATCTTATGTTGGCGGGGGCAAACTGAGGTATTGAGG160.1335559265442404No Hit
TGCCATCTTGCCACATCTACGTCCTTGGGCGAAAAGGGTTGGACATCATT160.1335559265442404No Hit
TGCCATCTTGCCACATCTCTCAGGACCGACTGACCCATGTTCAACTGCTG150.12520868113522537No Hit
TGCCATCTTGCCACATCTTAGGAACGAAAGCAAGGGTATCCGCCAACTTG150.12520868113522537No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCCCCATGTACTCTGCGTTGA150.12520868113522537No Hit
TGCCATCTTGCCACATCTGAAGCAGTGGTATCAACGCAGAGTACATGGGG150.12520868113522537Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTCCCCAAGGGTATCCGCCAACTTGAGGAGACC150.12520868113522537No Hit
TGCCATCTTGCCACATCTTCCGCGCCCCGCCAAGGGTATCCGCCAACTTG140.11686143572621034No Hit
TGCCATCTTGCCACATCTTTGTGTTGGCGGATACACTGCTTCCCATGTAC140.11686143572621034No Hit
TGCCATCTTGCCACATCTCTCAGGACCGACTCCCATGTACTCTGCGTTGA140.11686143572621034No Hit
GAGGACATCTTATGGTGCACTGGGGCAAACTGAGGTATTGAGGAGACCAA130.10851419031719532No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGTCCCATGTACTCTGCGTT130.10851419031719532No Hit
TGCCATCTTGCCACATCTCTGTGTTGGCGGATACCCATGTACTCTGCGTT130.10851419031719532No Hit
GAGGACATCTTATGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC130.10851419031719532No Hit
TGCCATCTTGCCACATCTTAGGGTATCCGCCAACTTGAGGAGACCAAGTC130.10851419031719532No Hit
TGCCATCTTGCCACATCTACTCTGTGTTGGCGGATACCCATGTACTCTGC130.10851419031719532No Hit
TGCCATCTTGCCACATCTTGCAAGGGTATCCGCCAACTTGAGGAGACCAA130.10851419031719532No Hit
TGCCATCTTGCCACATCTTCGTCCTTGGGCGAAAAGGGTTGGACATCATC130.10851419031719532No Hit
TGCCATCTTGCCACATCTTGAGAAGGGTATCCGCCAACTTGAGGAGACCA130.10851419031719532No Hit
TGCCATCTTGCCACATCTTGAAGCAGTGGTATCAACGCAGAGTACATGGA130.10851419031719532Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTACCCTGGGAAGGGTATCCGCCAACTTGAGGAG120.10016694490818029No Hit
TGCCATCTTGCCACATCTTAGCCCAAGGACGTCCCATGTACTCTGCGTTG120.10016694490818029No Hit
TGCCATCTTGCCACATCTTCGGAAGGGGAAGGGTATCCGCCAACTTGAGG120.10016694490818029No Hit
TGCCATCTTGCCACATCTTAGAAGGGTATCCGCCAACTTGAGGAGACCAA120.10016694490818029No Hit
TGCCATCTTGCCACATCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCC120.10016694490818029No Hit
TGCCATCTTGCCACATCTGGGGGCAAACTGAGGTATTGAGGAGACCAAGT120.10016694490818029No Hit
TGCCATCTTGCCACATCTCCCGCGCCCCGCCAAGGGTATCCGCCAACTTG120.10016694490818029No Hit
TGCCATCTTGCCACATCTTAGGCGAAAAGGGTTGGACATCATCCGGGAAG120.10016694490818029No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG120.10016694490818029No Hit
AAGGTGGATGGTGATGTGGGAGGAGGAAGGCGGGTCCGGAAGGGGAAGGG120.10016694490818029No Hit
AAGGTGGATGGTGATGTGGTGGAGGAGGAAGGCGGGTCCGGAAGGGGAAG120.10016694490818029No Hit
TGCCATCTTGCCACATCTTAGGGCGAAAAGGGTTGGACATCATCCGGAAG120.10016694490818029No Hit
TGCCATCTTGCCACATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC120.10016694490818029No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)