FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39115
Total Bases5.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1728844.19787805189825No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA817020.8871277003707No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA400610.24159529592228No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC12833.2800715837913845No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT8422.152626869487409No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG5871.5007030550939537No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG4931.26038604116068No Hit
TGCCATCTTGCCACATCTGATAGCTGCCCGGCATGAGCCAGAGGCAATGA1960.5010865396906558No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT1290.32979675316374796No Hit
TGCCATCTTGCCACATCTGCTTTCTAAATCTCAAGTGAGTGCTTCTATTT1030.2633260897353956No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGACTGA850.2173079381311517No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT740.18918573437300268No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC650.16617665857088074No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT530.13549789083471814No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG520.1329413268567046No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT520.1329413268567046No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC510.13038476287869105No Hit
TGCCATCTTGCCACATCTTGTAGCGGATTGCTCCTTTAATTACTTCTTCA500.12782819890067748No Hit
GAGGACATCTTATGGTGTACTGAAATGGAAAGGGTATTTGGTTTCCCAGT500.12782819890067748No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTCCCCAGT490.12527163492266394No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT480.1227150709446504No Hit
TGCCATCTTGCCACATCTTTCCCTCGCGAAGGGTATCCGCCAACTTGAGG480.1227150709446504No Hit
TGCCATCTTGCCACATCTTCTTCCATTCCTCTTCCTCCTTTGTCTGGAAT450.11504537901060974No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT440.11248881503259618No Hit
TGCCATCTTGCCACATCTACGTCCTTGGGCAAAGACCAGCATTTTCCATA440.11248881503259618No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGCTTCCCAGT420.10737568707656908No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT400.102262559120542No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)