FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39115
Total Bases5.6 Mbp
Sequences flagged as poor quality0
Sequence length35-145
%GC58

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA1645042.05547743832289No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC783420.02812220375815No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC38309.791640035791897No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG18964.84724530231369No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC5741.4674677233797775No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC4841.2373769653585582No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1410.36047552089991053No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT1280.3272401891857344No Hit
GTTGGCGGATACCCTTCCACCCATCGTGTCAGGAGGCCTCCGTGTCCATG1260.3221270612297073No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1100.28122203758149045No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG950.24287357791128722No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG930.23776044995526013No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT760.1942988623290298No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG750.19174229835101625No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA720.1840726064169756No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA710.18151604243896202No Hit
GTTGGCGGATACCCTTCTTCTAGAAAGCTTTCTAGCCTGCAGAGGCTGGT690.17640291448293494No Hit
GTTGGCGGATACCCTTCCATGAGTTATGGAAAATGCTGGTCTTTGCCCAA680.1738463505049214No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA630.16106353061485365No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA550.14061101879074522No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG540.13805445481273168No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG540.13805445481273168No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT540.13805445481273168No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA510.13038476287869105No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC510.13038476287869105No Hit
GTTGGCGGATACCCTTCCGAAAGGAAGATTAATACCGCATAACATTACAT490.12527163492266394No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG480.1227150709446504No Hit
GTTGGCGGATACCCTTCGCGAGGGAAAGATGTGGCAAGATGGCAAACTGG470.12015850696663684No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG460.11760194298862328No Hit
TACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATACTCCTTCA450.11504537901060974No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATACTCCTTCA450.11504537901060974No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG420.10737568707656908No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATAC420.10737568707656908No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT400.102262559120542No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)