FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37828
Total Bases5.4 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC2093455.33995981812414No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA471512.464312149730358No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG30217.98614782700645No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC5971.5781960452574813No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC5281.3957914772126467No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC2820.7454795389658454Clontech Universal Primer Mix Long (96% over 26bp)
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC2180.5762926932431004No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT1820.4811250925240563No Hit
TACCTCAGTTTGCCCCCATGGGGGGGAAGCAGTGGTATCAACGCAGAGTA1370.36216559162525114Clontech Universal Primer Mix Long (96% over 27bp)
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT1350.35687850269641536No Hit
TACCTCAGTTTGCCCCCAACACAGGGAAGCAGTGGTATCAACGCAGAGTA1250.33044305805223645Clontech Universal Primer Mix Long (96% over 26bp)
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA950.2511367241196997No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT870.22998836840435657No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA850.2247012794755208No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA780.20619646822459553No Hit
GTGTCGCTACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATAC690.18240456804483451No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCATGTACTCTGCGTTGA680.17976102358041662No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC610.1612562123294914No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA570.1506820344718198No Hit
GTTGGCGGATACCCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC560.1480384900074019Clontech SMART CDS Primer II A (96% over 26bp)
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG530.14010785661414824No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACCCACGCAAAATAC510.13482076768531248No Hit
GTTGGCGGATACCCTTGCTCTGTGTTGGCGGATACCCATGTACTCTGCGT510.13482076768531248No Hit
TACCTCAGTTTGCCCCCATGTACTGTGCGTGGGAAGCAGTGGTATCAACG480.1268901342920588No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG470.1242465898276409No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC470.1242465898276409No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACGCACACGCAAAATAC460.12160304536322301No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAT440.11631595643438722No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG430.11367241196996933No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATAC420.11102886750555144No Hit
GTTGGCGGATACCCTTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT410.10838532304113355Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTCCATGAGTTATGGAAAATGCTGGTCTTTGCCCCC390.10309823411229777No Hit
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCCATGTACTCTGCGTTG390.10309823411229777No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA380.10045468964787987No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC380.10045468964787987No Hit
TACCTCAGTTTGCCCCCATGGGAAGCAGTGGTATCAACGCAGAGTACATG380.10045468964787987Clontech Universal Primer Mix Long (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)