FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21841
Total Bases3.1 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA938042.94675152236619No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA309914.188910764159148No Hit
TGCCCTCTCTGCCTTTTCTCCCCCGGGGTATTTGGTTTCCCAGTCCACTA11545.283640858935031No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT4321.9779314133968224No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2791.2774140378187813No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2241.0255940662057599No Hit
TGCCATCTTGCCACATCTTTTCTGCGGGATGAACCGAACGCCGGGTTAAG2110.9660729820063184No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT1670.7646170047159012No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT1440.6593104711322741No Hit
AAGGTGGATGGTGATGTGGTGGGGGAGGAGGAAGGCGGGTCCGGAAGGGG1120.5127970331028799No Hit
AAGGTGGATGGTGATGTGGTGGAGGAGGAAGGCGGGTCCGGAAGGGGAAG1070.489904308410787No Hit
TGCCATCTTGCCACATCTACGTCCTTGGGCGAAAAGGGTTGGACATCATC1070.489904308410787No Hit
TGCCATCTTGCCACATCTTTCTCCAAGCAGTGGTATCAACGCAGAGTACA1030.47159012865711275Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTCCTCTCTGCTCCGAAGCACTGTGGAAGAAGG990.4532759489034385No Hit
TGCCATCTTGCCACATCTTATCTGAGGTCGCGAAGGGTATCCGCCAACTT970.44411885902660136No Hit
TGCCATCTTGCCACATCTTCCGAGGCAGAGCCAACGCTTCCCAGGGGCAA840.3845977748271599No Hit
TGCCATCTTGCCACATCTTAGGGCGAAAAGGGTTGGACATCATCCCAAGC750.3433908703813928No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC730.33423378050455566No Hit
TGCCATCTTGCCACATCTTAGGCGAAAAGGGTTGGACATCATCCCAAGCA670.30676251087404427No Hit
TGCCATCTTGCCACATCTTCCTCCTTTCGAAGGGTATCCGCCAACTTGAG580.2655556064282771No Hit
TGCCATCTTGCCACATCTGCACCCGGGTGGAGCCGCCGCAGGTGCAGATC570.2609770614898585No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT560.25639851655143997No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT510.2335057918593471No Hit
TGCCATCTTGCCACATCTTCTCCCCTACGGTGTCCACTACTAAGTACTAA480.2197701570440914No Hit
TGCCATCTTGCCACATCTTGAGAGTGGAAGGGTATCCGCCAACTTGAGGA470.21519161210567284No Hit
TGCCATCTTGCCACATCTTGACCACCAGGGAAGGGTATCCGCCAACTTGA450.2060345222288357No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT440.20145597729041712No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT430.19687743235199856No Hit
TGCCATCTTGCCACATCTTCCTCCCCAAGCAGTGGTATCAACGCAGAGTA430.19687743235199856Clontech Universal Primer Mix Long (96% over 25bp)
TGCCATCTTGCCACATCTTGATTCGAAGGGTATCCGCCAACTTGAGGAGA370.16940616272148712No Hit
TGCCATCTTGCCACATCTTCCTTGGGCGAAAAGGGTTGGACATCATCCCA350.16024907284464998No Hit
TGCCATCTTGCCACATCTGTGAAAAGTTTGGGAAGGGTATCCGCCAACTT350.16024907284464998No Hit
TGCCATCTTGCCACATCTCGAGAGTGGAAGGGTATCCGCCAACTTGAGGA340.1556705279062314No Hit
TGCCATCTTGCCACATCTTGTCCTTGGGCGAAAAGGGTTGGACATCATCC340.1556705279062314No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG340.1556705279062314No Hit
AAGGTGGATGGTGATGTGGGAGGAGGAAGGCGGGTCCGGAAGGGGAAGGG320.14651343802939426No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGCTTCCCAGTCCACTA310.14193489309097568No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC310.14193489309097568No Hit
TGCCATCTTGCCACATCTAGACCACCAGGGAAGGGTATCCGCCAACTTGA300.13735634815255712No Hit
TGCCATCTTGCCACATCTGCGAAAAGGGTTGGACATCATCCCAAGCAGTG280.12819925827571999No Hit
TGCCATCTTGCCACATCTTCGCCACTCCGGAAGGGTATCCGCCAACTTGA280.12819925827571999No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT280.12819925827571999No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT270.1236207133373014No Hit
TGCCATCTTGCCACATCTCGTCCTTGGGCGAAAAGGGTTGGACATCATCC270.1236207133373014No Hit
TGCCATCTTGCCACATCTTGTCTGACAGATTCAAGGGTATCCGCCAACTT260.11904216839888283No Hit
TGCCATCTTGCCACATCTGTACCAGCTGCTAATGCGGCTCAGCAAGGGTA240.1098850785220457No Hit
TGCCATCTTGCCACATCTACGTCCTTGGGCGAAAAGGGTTGGACATCATT240.1098850785220457No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG230.10530653358362713No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)