FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21841
Total Bases3.1 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC1002245.886177372830915No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC294113.465500663889015No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG3751.716954351906964No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2751.259099858065107No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2211.0118584313905041No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT1850.8470308136074356No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT1560.714253010393297No Hit
GTTGGCGGATACCCTTCCATGGCCACCGTCCTGCTGTCTATATCAACCAA1500.6867817407627856No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA1410.6455748363170185No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC980.44869740396501995Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGGATACCCTTCCCCTTCCGGACCCGCCTTCCTCCTCCACCACAT980.44869740396501995No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATAAGATGTGGCAAGATGGCAAA970.44411885902660136No Hit
GTTGGCGGATACCCTTCCCCTTCCGGACCCGCCTTCCTCCTCCCCCACCA940.43038322421134567No Hit
GTTGGCGGATACCCTTCCATAGCCACCGTCCTGCTGTCTATATCAACCAA870.39833340964241565No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT750.3433908703813928No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA730.33423378050455566No Hit
GTTGGCGGATACCCTTCGAAAGGAGGAAGATGTGGCAAGATGGCAAACTG600.27471269630511425No Hit
GTTGGCGGATACCCTTCCACCTTGCCCTTCTTCCACAGTGCTTCGGAGCA590.27013415136669566No Hit
GTTGGCGGATACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATA570.2609770614898585No Hit
GTTGGCGGATACCCTTCCACTCTCAAGATGTGGCAAGATGGCAAACTGGT470.21519161210567284No Hit
GTTGGCGGATACCCTTCCCTGGTGGTCAAGATGTGGCAAGATGGCAAACT460.21061306716725425No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT420.19229888741357995No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTGGGATGATG400.1831417975367428Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTT400.1831417975367428No Hit
GTTGGCGGATACCCTTCGAATCAAGATGTGGCAAGATGGCAAACTGGTGT370.16940616272148712No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACCCACGCAAAATAC350.16024907284464998No Hit
GTTGGCGGATACCCTTCCCAAACTTTTCACAGATGTGGCAAGATGGCAAA340.1556705279062314No Hit
GTTGGCGGATACCCTTCCGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT340.1556705279062314No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCATGTACTCTGCGTTGA340.1556705279062314No Hit
GTTGGCGGATACCCTTGCTCTGTGTTGGCGGATACCCATGTACTCTGCGT330.15109198296781284No Hit
GTTGGCGGATACCCTTCCACTCTCGAGATGTGGCAAGATGGCAAACTGGT330.15109198296781284No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG330.15109198296781284No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG310.14193489309097568No Hit
GTTGGCGGATACCCTTCCCTGGTGGTCTAGATGTGGCAAGATGGCAAACT300.13735634815255712No Hit
GTGTCGCTACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACT280.12819925827571999No Hit
GTTGGCGGATACCCTTCCCCTTCCGGACCCGCCTTCCTCCTCCCACATCA270.1236207133373014No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT270.1236207133373014No Hit
GTTGGCGGATACCCTTCCGGAGTGGCGAAGATGTGGCAAGATGGCAAACT270.1236207133373014No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATAC250.11446362346046426No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC250.11446362346046426No Hit
GTTGGCGGATACCCTTGAATCTGTCAGACAAGATGTGGCAAGATGGCAAA250.11446362346046426No Hit
GTGTCGCTACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATAC240.1098850785220457No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC240.1098850785220457No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC230.10530653358362713No Hit
CGAATAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT230.10530653358362713No Hit
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCCTTCCTGTGTTGGCGG220.10072798864520856No Hit
GTTGGCGGATACCCTTCCGGAGTGGCGGAGATGTGGCAAGATGGCAAACT220.10072798864520856No Hit
CGAAGTAGACCGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT220.10072798864520856No Hit
GTGTCGCTACCTCAGTTTGCACCCATGTCCCTTACACACACGCAAAATAC220.10072798864520856No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAGTAC220.10072798864520856No Hit
GTTGGCGGATACCCCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT220.10072798864520856Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTGCTGAGCCGCATTAGCAGCTGGTACAGATGTGGC220.10072798864520856No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)