FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26997
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA1045638.73022928473534No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC563520.872689558099047No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT12914.782012816238841No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG5472.026151053820795No Hit
TGCCATCTTGCCACATCTATGATGTCCAACCCTTTTCGAAGCAGTGGTAT4431.6409230655257991No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG4091.5149831462755121No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA3411.263103307774938No Hit
TGCCATCTTGCCACATCTACGTCCTTGGGCGAAAAGGGTTGGACATCATC2550.944549394377153No Hit
TGCCATCTTGCCACATCTACTCTGCGTTGATACCACTGCTTCCCATGTAC1840.6815572100603771Clontech Universal Primer Mix Long (96% over 26bp)
TGCCATCTTGCCACATCTTAGATGTCCAACCCTTTTCGAAGCAGTGGTAT1340.49635144645701373No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT1030.3815238730229285No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG910.33707448975812127No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT780.2889209912212468No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT710.26299218431677596No Hit
TGCCATCTTGCCACATCTTTGATGTCCAACCCTTTTCGAAGCAGTGGTAT700.2592880690447087No Hit
TGCCATCTTGCCACATCTTAGAAGGGTATCCGCCAACTTGAGGAGACCAA680.2518798385005741No Hit
TGCCATCTTGCCACATCTTAGGGCGAAAAGGGTTGGACATCATCCGGGAA660.24447160795643957No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG650.24076749268437236No Hit
TGCCATCTTGCCACATCTTCGCCCAAGGACGTCCCATGTACTCTGCGTTG620.22965514686817054No Hit
TGCCATCTTGCCACATCTTAGGAAGCATTCACTACTCCCGTGTGCCCCGC610.22595103159610327No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC570.2111345705078342No Hit
TGCCATCTTGCCACATCTACGTCCTTGGGCGAAAAGGGTTGGACATCATT570.2111345705078342No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT560.20743045523576695No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTA550.20372633996369968No Hit
TGCCATCTTGCCACATCTTAGGGCGAAAAGGGTTGGACATCATCGGGGAA530.19631810941956512No Hit
TGCCATCTTGCCACATCTCGTCCTTGGGCGAAAAGGGTTGGACATCATCC500.18520576360336333No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG500.18520576360336333No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT500.18520576360336333No Hit
TGCCATCTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT480.17779753305922882No Hit
TGCCATCTTGCCACATCTTAGGCGAAAAGGGTTGGACATCATCCGGGAAG450.166685187243027No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC450.166685187243027No Hit
TGCCATCTTGCCACATCTCGTCCTTGGGCGAAAAGGGTTGGACATCATCG440.16298107197095973No Hit
TGCCATCTTGCCACATCTTAGCAGGGTATCCGCCAACTTGAGGAGACCAA440.16298107197095973No Hit
TGCCATCTTGCCACATCTTAGGCGAAAAGGGTTGGACATCATCGGGGAAG440.16298107197095973No Hit
TGCCATCTTGCCACATCTGTGCCATCTTGCAAGGGTATCCGCCAACTTGA430.15927695669889247No Hit
TGCCATCTTGCCACATCTTGTCCTTGGGCGAAAAGGGTTGGACATCATCG420.1555728414268252No Hit
TGCCATCTTGCCACATCTTAGGGCGAAAAGGGTTGGACATCATCGAAAAG380.14075638033855614No Hit
TGCCATCTTGCCACATCTTGCCAAGGGTATCCGCCAACTTGAGGAGACCA370.13705226506648885No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT370.13705226506648885No Hit
TGCCATCTTGCCACATCTTGTCCTTGGGCGAAAAGGGTTGGACATCATCC370.13705226506648885No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT350.12964403452235435No Hit
TGCCATCTTGCCACATCTTAGTACATGGGGGCAAACTGAGGTATTGAGGA330.12223580397821979No Hit
TGCCATCTTGCCACATCTTCCTTGGGCGAAAAGGGTTGGACATCATCGGG320.11853168870615255No Hit
TGCCATCTTGCCACATCTTATCTGAGGTCGCGAAGGGTATCCGCCAACTT320.11853168870615255No Hit
TGCCATCTTGCCACATCTTAGGGCGAAAAGGGTTGGACATCATCCCTTCC310.11482757343408527No Hit
TGCCATCTTGCCACATCTTTCACTCAACCTCCAGAATTATCTTCCTAAAA290.10741934288995073No Hit
TGCCATCTTGCCACATCTACGTCCTTGGGCAAAGACCAGCATTTTCCATA280.10371522761788347No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACCA270.10001111234581621No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)