FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26997
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length35-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC1018837.73752639182131No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG619722.95440234100085No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC5442.015038708004593No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC4031.4927584546431085No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC3201.1853168870615254No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT2450.9075082416564804No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA2220.8223135903989333No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC1920.7111901322369153Clontech Universal Primer Mix Long (96% over 26bp)
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1660.6148831351631663No Hit
GTTGGCGGATACCCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC1400.5185761380894174Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1190.4407897173760047No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT1040.3852279882949957No Hit
GTTGGCGGATACCCTTCCATGAGTTATGGAAAATGCTGGTCTTTGCCCCC1010.3741156424787939No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCATGTACTCTGCGTTGA980.3630032966625921No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA940.34818683557432306No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA790.2926251064933141No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA780.2889209912212468No Hit
GTTGGCGGATACCCTTGCTCTGTGTTGGCGGATACCCATGTACTCTGCGT750.277808645405045No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA710.26299218431677596No Hit
TACCTCAGTTTGCCCCCATGGGAAGCAGTGGTATCAACGCAGAGTACATG700.2592880690447087Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA690.2555839537726414No Hit
GTTGGCGGATACCCTTCTAAGATGTGGCAAGATGGCAAACTGGTGTAGAA670.24817572322850687No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA660.24447160795643957No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG660.24447160795643957No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA610.22595103159610327No Hit
TACCTCAGTTTGCCCCCGCAGAGTACATGGGAAGCAGTGGTATCAACGCA600.22224691632403598Clontech Universal Primer Mix Long (95% over 22bp)
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCCATGTACTCTGCGTTG590.21854280105196872No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG570.2111345705078342No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCAAACTGGTGTAGAACCAT550.20372633996369968No Hit
GTTGGCGGATACCCTTGGACATCATCCCATGTACTCTGCGTTGATACCAC500.18520576360336333Clontech SMART CDS Primer II A (95% over 21bp)
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC470.17409341778716153No Hit
GTTGGCGGATACCCTTCCAGTAGAAGCGGGGCACACGGGAGTAGTGAATG470.17409341778716153No Hit
GTTGGCGGATACCCTTGTACTCTGCGTTGATACCACTGCTTCCCATGTAC450.166685187243027Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGGATACCCTGCTAAGATGTGGCAAGATGGCAAACTGGTGTAGAA450.166685187243027No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT440.16298107197095973No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA420.1555728414268252No Hit
GTTGGCGGATACCCTTGTACTCTGCGTTGATACCACCCCATGTACTCTGC400.14816461088269067Clontech SMART CDS Primer II A (95% over 21bp)
GTTGGCGGATACCCTTGCAAGATGGCACAGATGTGGCAAGATGGCAAACT400.14816461088269067No Hit
GTTGGCGGATACCCTTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT390.1444604956106234Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTCCATGAGTTATGGAAAATGCTGGTCTTTGCCCAA380.14075638033855614No Hit
GTTGGCGGATACCCTTGGCAAGATGTGGCAAGATGGCAAACTGGTGTAGA370.13705226506648885No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCGGATGA360.1333481497944216Clontech Universal Primer Mix Long (96% over 26bp)
TACCTCAGTTTGCCCCCATGTACTAAGATGTGGCAAGATGGCAAACTGGT340.12593991925028705No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATAAGATGTGGCAAGATGGCAAA320.11853168870615255No Hit
GTTGGCGGATACCCTTGCTGTGTTGGCGGATACCCATGTACTCTGCGTTG300.11112345816201799No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC300.11112345816201799No Hit
GTTGGCGGATACCCTTGCTCCCATGTACTCTGCGTTGATACCACTGCTTC300.11112345816201799Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCCTTCCTGTGTTGGCGG290.10741934288995073No Hit
GTTGGCGGATACCCTTCCGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT290.10741934288995073No Hit
GTTGGCGGATACCCCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT290.10741934288995073Clontech SMART CDS Primer II A (96% over 26bp)
GTGTCGCTACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACT290.10741934288995073No Hit
TACCTCAGTTTGCCCCCATGGGGAAGCAGTGGTATCAACGCAGAGTACAT280.10371522761788347Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGGCGGATACCCTTCTTCCCATGTACTCTGCGTTGATACCACTGCTTC280.10371522761788347Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTGCCTGTGTTGGCGGATACCCATGTACTCTGCGTT270.10001111234581621No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)