FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20005
Total Bases2.8 Mbp
Sequences flagged as poor quality0
Sequence length35-145
%GC56

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA735336.75581104723819No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC433721.679580104973756No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG13926.958260434891277No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC7533.764058985253686No Hit
TACCTCAGTTTGCCCCCATGGGAAGCAGTGGTATCAACGCAGAGTACATG1840.9197700574856286Clontech Universal Primer Mix Long (96% over 26bp)
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1630.8147963009247688No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1060.5298675331167209No Hit
GTTGGCGGATACCCTTAAGCAAGATGTGGCAAGATGGCAAACTGGTGTAG1060.5298675331167209No Hit
GTTGGCGGATACCCTTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT920.4598850287428143Clontech SMART CDS Primer II A (96% over 26bp)
TACCTCAGTTTGCCCCCATGGAAGCAGTGGTATCAACGCAGAGTACATGG820.4098975256185954Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT820.4098975256185954No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG700.34991252186953264No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC690.3449137715571107Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGGATACCCTTAAGCTAGATGTGGCAAGATGGCAAACTGGTGTAG580.2899275181204699No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCATGTACTCTGCGTTGA580.2899275181204699No Hit
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCCATGTACTCTGCGTTG580.2899275181204699No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG580.2899275181204699No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT550.2749312671832042No Hit
GTTGGCGGATACCCTTCCTCCTTCAAACTGTCTCCATGCCTAACTTTTCC430.21494626343414144No Hit
GTTGGCGGATACCCTTCATCCTTCAAACTGTCTCCATGCCTAACTTTTCC420.20994751312171958No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG420.20994751312171958No Hit
GTTGGCGGATACCCTTGCTCTGTGTTGGCGGATACCCATGTACTCTGCGT410.2049487628092977No Hit
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCCTTCCTGTGTTGGCGG400.1999500124968758No Hit
GTTGGCGGATACCCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC400.1999500124968758Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA390.19495126218445388No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA380.189952511872032No Hit
GTTGGCGGATACCCTTCCGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT380.189952511872032No Hit
TACCTCAGTTTGCCCCCATGCACCATAAGATGTCCTCAACTGGTGTAGAA380.189952511872032No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG370.1849537615596101No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG360.1799550112471882No Hit
GTTGGCGGATACCCTTCCATTCAAGATGTGGCAAGATGGCAAACTGGTGT350.17495626093476632No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA270.13496625843539115No Hit
TACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATACTCCTTCA270.13496625843539115No Hit
TACCTCAGTTTGCCCCCATGGGAAGCAGTGGTATCAACGCAGAGTAGATG270.13496625843539115Clontech Universal Primer Mix Long (96% over 26bp)
TACCTCAGTTTGCCCCCGTGCACCATAAGATGTCCTCAACTGGTGTAGAA240.11997000749812546No Hit
GTTGGCGGATACCCTTGTACTCTGCGTTGATACCACTGCTTCCCATGTAC240.11997000749812546Clontech Universal Primer Mix Long (96% over 26bp)
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG240.11997000749812546No Hit
GTTGGCGGATACCCTTGCAAGATGTGGCAAGATGGCAAACTGGTGTAGAA240.11997000749812546No Hit
TACCTCAGTTTGCCCCCATGCAGTGGTATCAACGCAGAGTACATGGGAAG240.11997000749812546Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG230.11497125718570357No Hit
GTTGGCGGATACCCTTCTAAGATGTGGCAAGATGGCAAACTGGTGTAGAA210.10497375656085979No Hit
GTTGGCGGATACCCTTCCTCTGTGTTGGCGGATACCCATGTACTCTGCGT210.10497375656085979No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT210.10497375656085979No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)