FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11169
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA416137.254901960784316No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT215419.285522428149342No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC9498.49673202614379No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG3272.9277464410421703No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG3182.8471662637657804No Hit
TGCCATCTTGCCACATCTTCCTCCCCAAGCAGTGGTATCAACGCAGAGTA2201.9697376667562Clontech Universal Primer Mix Long (96% over 25bp)
TGCCATCTTGCCACATCTTACATTTCTCCAACACAGCTGATGGAAAGAAG1651.4773032500671501No Hit
TGCCATCTTGCCACATCTCACATTTCTCCAACACAGCTGATGGAAAGAAG990.8863819500402902No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT960.8595218909481601No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG560.5013877697197601No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC470.42080759244337No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT350.31336735607485No Hit
TGCCATCTTGCCACATCTTCTGTCCTGGCACTGCACTCTGTGGGGCTTCC350.31336735607485No Hit
TGCCCTCTCTGCCTTTTCTCAAGCAGTGGTATCAACGCAGAGTACATGGG320.28650729698272Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTCCTCCCAAAGCAGTGGTATCAACGCAGAGTA290.25964723789059Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG270.24174053182917005No Hit
TGCCCTCTCTGCCTTTTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCG260.23278717879846003No Hit
TGCCATCTTGCCACATCTTCCTCCCTAAGCAGTGGTATCAACGCAGAGTA240.21488047273704003Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT230.20592711970633001No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT210.18802041364491No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG190.17011370758349No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC190.17011370758349No Hit
TGCCATCTTGCCACATCTTCATATCCAAATGACTACTAAACTCTGACAAA170.15220700152207No Hit
TGCCATCTTGCCACATCTTGTTTTCACTCAGGAACTTCATGCCATAGGGG160.14325364849136No Hit
GAGGACATCTTGCCACATCTTCCTCCCCAAGCAGTGGTATCAACGCAGAG150.13430029546065003Clontech Universal Primer Mix Long (95% over 24bp)
TGCCATCTTGCCACATCTTCCCATACGCTGAGGAACAGCCTGCCATCCGT140.12534694242994002No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT140.12534694242994002No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT140.12534694242994002No Hit
AAGGTGGATGGTGATGTGGTCAAGCAGTGGTATCAACGCAGAGTACATGG140.12534694242994002Clontech Universal Primer Mix Long (96% over 27bp)
TCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT130.11639358939923002No Hit
TGCCGTCTAACCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC130.11639358939923002No Hit
AAGGTGGATGGTGATGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT130.11639358939923002No Hit
GAGGACATCTTATGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC120.10744023636852001No Hit
TGCCATCTTGCCACATCTTAGTGGAAGGGTATCCGCCAACTTGAGGAGAC120.10744023636852001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)