FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44692
Total Bases6.4 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT2283251.08744294280856No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT839218.77740982726215No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC521611.670992571377429No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG6881.5394254005191086No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG5831.3044840239863957No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT3950.8838270831468719No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATCGAACTTTCTG2510.5616217667591515No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC2470.5526716190817149No Hit
AAGGTGGATGGTGATGTGGTCAAGCAGTGGTATCAACGCAGAGTACATGG1090.24389152421014945Clontech Universal Primer Mix Long (96% over 27bp)
TGCCATCTTGCCACATCTTAGAAGTTTTGAAATCATTGAACATGGTCTTG1010.22599122885527614No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA1000.22375369193591696No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT980.21927861809719862No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG750.1678152689519377No Hit
TGCCCTCTCTGCCTTTTCTCAAGCAGTGGTATCAACGCAGAGTACATGGG720.1611026581938602Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT710.15886512127450103No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT710.15886512127450103No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT620.1387272890002685No Hit
TTCTGAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT560.1253020674841135No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT520.1163519198066768No Hit
TGCCATCTTGCCACATCTCCTCTTAAACACCATTAATCACCTCTCTAGGG520.1163519198066768No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT510.11411438288731764No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGC510.11411438288731764No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC510.11411438288731764No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT500.11187684596795848No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT480.10740177212924013No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG460.1029266982905218No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG460.1029266982905218No Hit
TTCTGCGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT450.10068916137116263No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)