FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31820
Total Bases4.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA1736754.57888120678819No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG450414.154619736015084No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC23177.2815839094908865No Hit
GTTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGG7182.2564424890006287No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC6792.1338780641106223No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC5641.7724701445631679No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1650.518541797611565No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1330.4179761156505342No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1110.3488372093023256No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1020.32055311125078567No Hit
GTTGGCGGATACCCTTCCCATTTCAGTGCACCATAAGATGTCCTCAACTG1010.31741043368950345No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG990.31112507856693905No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG900.28284098051539913No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG760.23884349465744817No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG730.22941546197360152No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA710.22313010685103707No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG620.19484600879949718No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG610.19170333123821495No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA600.18856065367693275No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG580.18227529855436833No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA550.17284726587052168No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA540.16970458830923946No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG530.16656191074795726No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA520.16341923318667503No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA510.16027655562539284No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT470.147705845380264No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA440.13827781269641734No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG420.13199245757385292No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAGTACTCCTTCA410.1288497800125707No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG390.12256442489000628No Hit
GTTGGCGGATACCCTTTCCATTTCAGTGCACCATAAGATGTCCTCAACTG380.11942174732872408No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATACTCCTTCA380.11942174732872408No Hit
TACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATACTCCTTCA370.11627906976744186No Hit
GTTGGCGGATACCCTTGCAAAAACCGAGGAGCATGCCGAACAGGAAACTC360.11313639220615963No Hit
GTTGGCGGATACCCTTCCGTTAAAGCGCAGAGACAGCTTATCAAACAGTT360.11313639220615963No Hit
TACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATACTCCTTCA340.10685103708359522No Hit
GTTGGCGGATACCCATGTCCCTTACACACACGCAAAATACTCCTTCAGCG330.10370835952231301No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACGCACACGCAAAATACTCCTTCA320.1005656819610308No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA320.1005656819610308No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)