FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19931
Total Bases2.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA1159358.16567156690583No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA478424.002809693442376No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT5712.864883849280016No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT3651.8313180472630575No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC1440.7224925994681651No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC1390.6974060508755205No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT1380.6923887411569917No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT760.3813155386081983No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT490.2458481762079173No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT480.2408308664893884No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT440.22076162761527268No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT410.20570969845968592No Hit
AAGGTGGATGGTGATGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT400.200692388741157No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG390.1956750790226281No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG350.17560584014851235No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT310.15553660127439667No Hit
TGCCCTCTCTGCCTTTTCTCAAGCAGTGGTATCAACGCAGAGTACATGGG310.15553660127439667Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACCA250.12543274296322313No Hit
AAGGTGGATGGGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT230.11539812352616527No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGCTTCCCAGTCCACTA230.11539812352616527No Hit
TGCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT210.10536350408910741No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCGCTA210.10536350408910741No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)