FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29778
Total Bases4.2 Mbp
Sequences flagged as poor quality0
Sequence length135-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC1031434.636308684263554No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA891729.94492578413594No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG12804.298475384512056No Hit
GTTGGCGGATACCCTTCCTGCAGAACTGCGAGGACGAAGACAGCGACGAG4681.5716300624622204No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC3441.155215259587615No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2790.9369333064678622No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2530.849620525219961No Hit
GTTGGCGGATACCCTTCCACACACGCAAAATACTCCTTCAGCGGAGCGAA1860.6246222043119082No Hit
TACCTCAGTTTGCCCCCATGGGAAGCAGTGGTATCAACGCAGAGTACATG1510.5070857680166566Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA1410.47350392907515615No Hit
GTTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGG1320.4432802740278058No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT1240.41641480287460547No Hit
GTTGGCGGATACCCTTCCTACTTTCTCCTTCCTCTTCTAGTTCCTGGCCA1120.37611659614480486No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC1050.3526093088857546Clontech Universal Primer Mix Long (96% over 26bp)
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG810.27201289542615353No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCATGTACTCTGCGTTGA800.2686547115320035No Hit
GTTGGCGGATACCCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC780.2619383437437034Clontech SMART CDS Primer II A (96% over 26bp)
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT740.2485056081671032No Hit
GTTGGCGGATACCCTTGCTCTGTGTTGGCGGATACCCATGTACTCTGCGT710.23843105648465307No Hit
GTTGGCGGATACCCTTCCATACACGCAAAATACTCCTTCAGCGGAGCGAA620.20820740143730274No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA590.1981328497548526No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG570.19141648196655248No Hit
GTTGGCGGATACCCTTCCATGAGTTATGGAAAATGCTGGTCTTTGCCCCC560.18805829807240243No Hit
GTTGGCGGATACCCTTCGGGAGATGTGGCAAGATGGCAAACTGGTGTAGA550.1847001141782524No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT520.17462556249580227No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG510.17126737860165223No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG490.16455101081335213No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG470.15783464302505207No Hit
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCCATGTACTCTGCGTTG450.15111827523675198No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA430.14440190744845188No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA420.14104372355430184No Hit
GTGTCGCTACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATAC410.1376855396601518No Hit
GTGTCGCTACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACT380.12761098797770165No Hit
GTTGGCGGATACCCTTCGGAAGATGTGGCAAGATGGCAAACTGGTGTAGA370.1242528040835516No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACCCACGCAAAATAC350.11753643629525151No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC350.11753643629525151No Hit
GTTGGCGGATACCCTTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT340.11417825240110148Clontech SMART CDS Primer II A (96% over 26bp)
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG340.11417825240110148No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC330.11082006850695145No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATAAGATGTGGCAAGATGGCAAA310.10410370071865137No Hit
GTTGGCGGATACCCTTGGGAGATGTGGCAAGATGGCAAACTGGTGTAGAA300.10074551682450132No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)