FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8174
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG357943.78517249816491No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC133216.295571323709325No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC6117.4749204795693664No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1672.0430633716662587No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1611.9696598972351358No Hit
GTTGGCGGATACCCTTCATCCAGGCCATGTCGGCGAGGAGTCTGGGTAGA1441.7616833863469537No Hit
GTTGGCGGATACCCTTCCTCAATAAATAGATCAAGTTCCAAACATCTGAA1141.3946660141913383No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1031.2600929777342795No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1001.223391240518718No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT720.8808416931734768No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA660.8074382187423538No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA550.6728651822852949No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA520.6361634450697333No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA500.611695620259359No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA320.3914851969659897No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA310.37925128456080254No Hit
GTTGGCGGATACCCTTCCTCCAGGCCATGTCGGCGAGGAGTCTGGGTAGA300.36701737215561536No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA290.3547834597504282No Hit
GTTGGCGGATACCCTTCCTGTGGGGATAGTGAGTTGTAGTTAAGGGTAGG270.33031563494005384No Hit
GTTGGCGGATACCCTTCCCTAACTGTCCAAGTATGAGCATACACTGCCAC210.25691216050893073No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG180.2202104232933692No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT180.2202104232933692No Hit
GTTGGCGGATACCCTTCTACACTTATATTCAAAACATCTTAGTCATACGA170.20797651088818206No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG170.20797651088818206No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCCATAAAGGCTTTA140.1712747736726205No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT130.15904086126743333No Hit
GTTGGCGGATACCCTTGATGAAGGACGTGATAAGCTGCGAAAAGATGTGG120.14680694886224616No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC120.14680694886224616No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA100.12233912405187179No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCAAACTGGTGTAGAACCAT100.12233912405187179No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGGTTCG100.12233912405187179No Hit
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCCTTCCTGTGTTGGCGG90.1101052116466846No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC90.1101052116466846Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGGATACTCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG90.1101052116466846No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)