FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32784
Total Bases4.7 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA1450244.234992679355784No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG489314.924963396778917No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC351710.727794045876037No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC7702.3487066861883847No Hit
GTTGGCGGATACCCTTCCTCTCTTCTTCAGATGTGGCAAGATGGCAAACT6832.083333333333333No Hit
GTTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGG4521.378721327476818No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC3981.214006832601269No Hit
GTTGGCGGATACCCTTCCATTTCAAAGAACAGAATGCTAAGGAAGATGTG3351.0218399219131284No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2670.8144216691068814No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1320.40263543191800877No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1240.3782332845290386No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1070.3263787213274768No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1020.31112737920937045No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG920.2806246949731577No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA860.26232308443143No Hit
GTTGGCGGATACCCTTCCTCTCTTCTTAAGATGTGGCAAGATGGCAAACT790.24097120546608103No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG770.23487066861883846No Hit
TACCTCAGTTTGCCCCCATGATCCACTCTCTTTCCACCAGACCCCACCTC770.23487066861883846No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG760.23182040019521719No Hit
GTTGGCGGATACCCTTCCTCTCTTCCTAAGATGTGGCAAGATGGCAAACT750.2287701317715959No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT610.186066373840898No Hit
GTTGGCGGATACCCTTCCATCCAAAAGATGTGGCAAGATGGCAAACTGGT600.18301610541727673No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA600.18301610541727673No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG600.18301610541727673No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA580.17691556857003415No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG520.1586139580283065No Hit
GTTGGCGGATACCCTTCCATAGGGTCCAGTACAGAAAGAAGAAAAAAGAG510.15556368960468522No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG470.1433626159102001No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA450.13726207906295754No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA440.13421181063933627No Hit
TACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATACTCCTTCA420.1281112737920937No Hit
TACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATACTCCTTCA410.12506100536847242No Hit
GTTGGCGGATACCCTTCCCATTTCAGTGCACCATAAGATGTCCTCAACTG410.12506100536847242No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG400.12201073694485115No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA400.12201073694485115No Hit
GTTGGCGGATACCCTTGAGCCCAGGAGTTCGAGACCAGCCTGCACAAGAT390.11896046852122988No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAACACTCCTTCA380.11591020009760859No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG360.10980966325036604No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA330.10065885797950219No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA330.10065885797950219No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATACTCCTTCA330.10065885797950219No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)