FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39477
Total Bases5.6 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC57

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA1250031.664006890087897No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC958024.267294880563366No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC29777.541099880943334No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG28937.328317754641943No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC7121.8035818324594068No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC5741.4540111963928364No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT2040.5167565924462345No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT1880.476226663626922No Hit
GTTGGCGGATACCCTTGTGTAGATGTGGCAAGATGGCAAACTGGTGTAGA1480.3749018415786407No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT1460.36983560047622666No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC1440.3647693593738126Clontech Universal Primer Mix Long (96% over 26bp)
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA1130.2862426222863946No Hit
GTTGGCGGATACCCTTCCTGCTGCAGCTCATTGGGGTAAAGAAGAAGCAT1060.2685107784279454No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1010.25584517567191023No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT960.24317957291587508No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG930.23558021126225398No Hit
GTTGGCGGATACCCTTGCAAAAACCGAGGAGCATGCCGAACAGGAAACTC840.21278212630139068No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG810.2051827646477696No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA800.20264964409656255No Hit
CGAAGTAGCCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT800.20264964409656255No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA780.19758340299414848No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG730.18491780023811333No Hit
CGAAGTAGACCAGCGGCATCCCGGCCCCACCCTGGACCTTAGCTCAGCAC730.18491780023811333No Hit
CGAAGTGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT720.1823846796869063No Hit
CGAATAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT710.17985155913569928No Hit
GTTGGCGGATACCCTTGCTAAGATGTGGCAAGATGGCAAACTGGTGTAGA640.16211971527725003No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT640.16211971527725003No Hit
GTTGGCGGATACCCTTGCTCTGTGTTGGCGGATACCCATGTACTCTGCGT620.15705347417483598No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC590.14945411252121488No Hit
GTTGGCGGATACCCTTCCCGAGCTAAGATGTGGCAAGATGGCAAACTGGT590.14945411252121488No Hit
CGAAGTAGACCGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT580.14692099197000785No Hit
GTTGGCGGATACCCTTGCTGAGCCAGCATTAGCAGCTGGTACAGATGTGG570.14438787141880083No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG560.14185475086759378No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA550.13932163031638675No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG550.13932163031638675No Hit
GTTGGCGGATACCCTTCATGCTGCAGCTCATTGGGGTAAAGAAGAAGCAT540.13678850976517973No Hit
GTTGGCGGATACCCTTCTAAGATGTGGCAAGATGGCAAACTGGTGTAGAA510.12918914811155863No Hit
GTTGGCGGATACCCTTCGCTAAGATGTGGCAAGATGGCAAACTGGTGTAG510.12918914811155863No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCATGTACTCTGCGTTGA510.12918914811155863No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG450.11399042480431643No Hit
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCCATGTACTCTGCGTTG450.11399042480431643No Hit
GTTGGCGGATACCCTTCCATTTGATACCCCATGACTGTGCCATCCAACCA440.11145730425310942No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG440.11145730425310942No Hit
GTTGGCGGATACCCTTCCGTCAAGCTTTCAGATGTGGCAAGATGGCAAAC440.11145730425310942No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCCCCCTGGACCTTAGCTCAGCAC420.10639106315069534No Hit
CAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT400.10132482204828128No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)