FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17351
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT534130.782087487752868No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA384622.165869402339922No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC363220.932511094461415No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACTTGAGG6803.9190824736326437No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT3652.10362515128811No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2581.4869459973488561No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2181.2564117341939947No Hit
TGCCATCTTGCCACATCTGCTCGGAAGGGTATCCGCCAACTTGAGGAGAC2001.152671315774307No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG1550.8933202697250879No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACTTGAG830.47835859604633735No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT620.35732810789003516No Hit
TGCCATCTTGCCACATCTTCCCACCTGACTATGTTATTGACCTAGATCTT280.161373984208403No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT280.161373984208403No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT280.161373984208403No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC250.14408391447178837No Hit
TGCCATCTTGCCACATCTTCTCGGAAGGGTATCCGCCAACTTGAGGAGAC240.13832055789291683No Hit
TGCCGTCTAACCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC230.1325572013140453No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCGCCAACTTGAGGA220.12679384473517377No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG210.12103048815630224No Hit
TGCCATCTTGCCACATCTCCCCACCTGACTATGTTATTGACCTAGATCTT200.1152671315774307No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT190.10950377499855915No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG190.10950377499855915No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACACAGAA180.10374041841968763No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATCCGCCAACTTGAG180.10374041841968763No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)