FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16749
Total Bases2.4 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC59

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA592035.34539375485103No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC536132.00788106752642No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC15939.511015583019882No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC3862.304615200907517No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC3131.868768284673712No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG3051.8210042390590482No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG600.35823034210997673No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG450.2686727565824825No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG420.2507612394769837No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG390.23284972237148488No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT380.22687921666965194No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG350.2089676995641531No Hit
GTTGGCGGATACCCTTAAGCTAAGATGTGGCAAGATGGCAAACTGGTGTA310.1850856767568213No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT310.1850856767568213No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG290.17314466535315542No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT260.15523314824765658No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG240.14329213684399067No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACCCACGCAAAATAC210.12538061973849185No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG210.12538061973849185No Hit
GTTGGCGGATACCCTTCCAGTAACGTCCACGTCTACAGCAACGGCCGCAT200.11941011403665891No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG190.11343960833482597No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA180.10746910263299302No Hit
GTGTCGCTACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATAC180.10746910263299302No Hit
GTTGGCGGATACCCTTGGACATCAGATGTGGCAAGATGGCAAACTGGTGT170.10149859693116008No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT170.10149859693116008No Hit
GTTGGCGGATACCCTTCTAGTAACGTCCACGTCTACAGCAACGGCCGCAT170.10149859693116008No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)