FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5223
Total Bases752.8 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA223042.695768715297724No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC91017.42293700938158No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG88616.963430978364926No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC711.3593720084242773No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC621.187057246793031No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT320.6126747080222095No Hit
GTTGGCGGATACCCTTCGTCCTGGGAAACGGGGCGCGGCTGGAAAGGCGG280.5360903695194332No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC260.4977982002680452Clontech Universal Primer Mix Long (96% over 26bp)
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG250.47865211564235116No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA250.47865211564235116No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA220.421213861765269No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG140.2680451847597166No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA130.2488991001340226No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG120.22975301550832855No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG110.2106069308826345No Hit
GTTGGCGGATACCCTTCCCTGCAACAGAAAGACTGTAGCCTAGAATCTCT110.2106069308826345No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA100.19146084625694046No Hit
TACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATACTCCTTCA100.19146084625694046No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG100.19146084625694046No Hit
GTTGGCGGATACCCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC80.15316867700555237Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG80.15316867700555237No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT70.1340225923798583No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA60.11487650775416428No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG60.11487650775416428No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG60.11487650775416428No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG60.11487650775416428No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)