FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17020
Total Bases2.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1130266.4042303172738No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC12287.215041128084606No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA11496.750881316098707No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT4442.608695652173913No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG4222.4794359576968272No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG3271.9212690951821387No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA2571.509988249118684No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC310.18213866039952997No Hit
GAGGACATCTTATGGTGTACTGAAATGGAAAGGGTATTTGGTTTCCCAGT290.17038777908343128No Hit
TGCCATCTTGCCACATCTACACATAACCTCAGGAATCAGCCCTACACAAC230.13513513513513514No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG230.13513513513513514No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT230.13513513513513514No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG220.12925969447708577No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT220.12925969447708577No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT210.12338425381903642No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTACTTGGTTTCCCAGT200.11750881316098707No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG200.11750881316098707No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT190.11163337250293771No Hit
GAGGACATCTTATGGTGCATTGAAATGGAAAGGGTATTTGGTTTCCCAGT180.10575793184488837No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT180.10575793184488837No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)