FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7756
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length136-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA241731.162970603403817No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC155019.98452810727179No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2413.107271789582259No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2102.707581227436823No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG861.10881897885508No Hit
TGCCATCTTGCCACATCTTCCTCCCCAAGCAGTGGTATCAACGCAGAGTA781.0056730273336771Clontech Universal Primer Mix Long (96% over 25bp)
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT590.7607013924703455No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT480.6188757091284167No Hit
TGCCATCTTGCCACATCTTAGCCTCCCAAAGTGCTAGAATTACAGGCATG450.5801959773078907No Hit
TGCCATCTTGCCACATCTTGACTTCTGGAAGGGTATCCGCCAACTTGAGG440.5673027333677153No Hit
TGCCATCTTGCCACATCTTATCTGAGGTCGCGAAGGGTATCCGCCAACTT420.5415162454873645No Hit
TGCCATCTTGCCACATCTTGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTC370.4770500257864879No Hit
TGCCATCTTGCCACATCTTAGCCCTCTTCTCAACAAGCAGTGGTATCAAC360.46415678184631254No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG350.45126353790613716No Hit
TGCCATCTTGCCACATCTGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTC330.4254770500257865No Hit
TGCCATCTTGCCACATCTTAGGGTATCCGCCAACTTGAGGAGACCAAGTC320.4125838060856112No Hit
TGCCATCTTGCCACATCTTCCTCCTCCTCCACAAGGGTATCCGCCAACTT280.36101083032490977No Hit
TGCCATCTTGCCACATCTTAGTGACCCATGGGGGCAAACTGAGGTATTGA270.3481175863847344No Hit
TGCCATCTTGCCACATCTTAGTCCCTCTCCTCCCACCTCCCTCTTCCTTC270.3481175863847344No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC270.3481175863847344No Hit
TGCCATCTTGCCACATCTCTGGGAAGGGTATCCGCCAACTTGAGGAGACC270.3481175863847344No Hit
TGCCATCTTGCCACATCTTGCCAAGGGTATCCGCCAACTTGAGGAGACCA230.296544610624033No Hit
TGCCATCTTGCCACATCTTCTACATCAGCATTTGCTGCTTCACATTGCAC210.2707581227436823No Hit
TGCCGTCTAACCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC190.2449716348633316No Hit
TGCCATCTTGCCACATCTTCCTCCCTAGTCAGTCTCTAGCAGGAGTGATA190.2449716348633316No Hit
TGCCATCTTGCCACATCTTGCAAGGGTATCCGCCAACTTGAGGAGACCAA190.2449716348633316No Hit
TGCCATCTTGCCACATCTTCTGCCTCCTCACTCATGGGGGCAAACTGAGG190.2449716348633316No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG180.23207839092315627No Hit
TGCCATCTTGCCACATCTGTGCCATGGGGGCAAACTGAGGTATTGAGGAG170.21918514698298092No Hit
TGCCATCTTGCCACATCTTAGCCTCCAAAAGTGTTGGGATTACAGGTGTG170.21918514698298092No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT160.2062919030428056No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT150.19339865910263024No Hit
TGCCATCTTGCCACATCTTCCTCCCTAAGCAGTGGTATCAACGCAGAGTA150.19339865910263024Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGCCATCTTGCCACATCTTACCCATGAATGGCGGCCCTAGCCTTCTACTT150.19339865910263024No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT140.18050541516245489No Hit
TGCCATCTTGCCACATCTGTTGGAACAATGTAGGGAAGGGTATCCGCCAA140.18050541516245489No Hit
TGCCATCTTGCCACATCTTTTCCCACTTAACCATGACTTTGGGACCTTAG140.18050541516245489No Hit
TGCCATCTTGCCACATCTTAGAAGTTGGAAGGGTATCCGCCAACTTGAGG120.15471892728210418No Hit
TGCCATCTTGCCACATCTACGTCCAAGGGTATCCGCCAACTTGAGGAGAC120.15471892728210418No Hit
TGCCATCTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT120.15471892728210418No Hit
GAGGACATCTTATGGTGCACTGATCCATTCCAATTTGAACAGGAGGGTTG120.15471892728210418No Hit
TGCCATCTTGCCACATCTTAGGGAAGGGTATCCGCCAACTTGAGGAGACC120.15471892728210418No Hit
GAGGACATCTTATGGTGCATGGGGGCAAACTGAGGTATTGAGGAGACCAA110.14182568334192883No Hit
TGCCATCTTGCCACATCTTAGTCATTTTCTTGTCAGTGGAGAAGCAGTGG110.14182568334192883No Hit
TGCCATCTTGCCACATCTTGACTTATGGAAGGGTATCCGCCAACTTGAGG110.14182568334192883No Hit
GAGGACATCTTATGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC110.14182568334192883No Hit
TGCCATTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT100.12893243940175347No Hit
TGCCATCTTGCCACATCTCGACTTCTGGAAGGGTATCCGCCAACTTGAGG100.12893243940175347No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT100.12893243940175347No Hit
GAGGACATCTTATGGTGCACTGAAGCAGTGGTATCAACGCAGAGTACATG100.12893243940175347Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT100.12893243940175347No Hit
TGCCATCTTGCCACATCTTAGGGTATCCGCCAACACAGAGTACATGGGAA100.12893243940175347No Hit
TGCCATCTTGCCACATCTTGCGTGGAAGTGATGGAAGGGTATCCGCCAAC100.12893243940175347No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGATATTTGGTTTCCCAGTCCACTA100.12893243940175347No Hit
TGCCATCTTGCCACATCTTAGGGGACCGAGATCCCAACTTAGAGCCCTTT90.11603919546157813No Hit
TGCCATCTTGCCACATCTTAGAAGGGTATCCGCCAACTTGAGGAGACCAA90.11603919546157813No Hit
TGCCATCTTGCCACATCTTAGCCCAAGCAGTGGTATCAACGCAGAGTACA90.11603919546157813Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT90.11603919546157813No Hit
TGCCATCTTGCCACATCTTAGCCATCCTTTCCTCTGTACAGGGTAAGCAG90.11603919546157813No Hit
TGCCATCTTGCCACATCTTTGGGAAGGGTATCCGCCAACTTGAGGAGACC80.1031459515214028No Hit
TGCCATCTTGCCACATCTATTTAAGGAAGCAGTGGTATCAACGCAGAGTA80.1031459515214028Clontech Universal Primer Mix Long (96% over 25bp)
TGCCATCTTGCCACATCTCTATAATAAACTGTCTGTCCTATGATAAAGTA80.1031459515214028No Hit
TGCCATCTTGCCACATCTACTCCAGATGAGCTGATCGTTATAGAGAAAAG80.1031459515214028No Hit
GAGGACATCTTATGGTGCACTAAGCAGTGGTATCAACGCAGAGTACATGG80.1031459515214028Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTATGGACTGCACATACTCCTCAAAAGCAGTGGT80.1031459515214028No Hit
TGCCATCTTGCCACATCTACACGACTACACTTTTGGAGCCCCCAAGCAGT80.1031459515214028No Hit
TGCCATCTTGCCACATCTTTCAACTTTTACATTCCTTACTATTAAGGCAA80.1031459515214028No Hit
TGCCGTCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC80.1031459515214028No Hit
TGCCATCTTGCCACATCTCTCGGCCGATCGAAAGGGAGTCGGGTTCAGAT80.1031459515214028No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTCCCCAGTCCACTA80.1031459515214028No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)