FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7756
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length35-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC234530.234657039711195No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG150819.443011861784427No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2403.094378545642084No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2102.707581227436823No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT1391.7921609076843734No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC650.8380608561113976Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA590.7607013924703455No Hit
GTTGGCGGATACCCTTCCCAAAGAAAAGAGAACTCTCCCCGGGGCTCCCG550.7091284167096441No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA470.6059824651882414No Hit
GTTGGCGGATACCCTTCCAGAAGTCAAGATGTGGCAAGATGGCAAACTGG440.5673027333677153No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA420.5415162454873645No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATAAGATGTGGCAAGATGGCAAA410.5286230015471893No Hit
GTTGGCGGATACCCTTCCCTCTCTGCTCCCTGCACAAAAAACCCAAAAAA390.5028365136668386No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG350.45126353790613716No Hit
GTTGGCGGATACCCTAAGATGTGGCAAGATGGCAAACTGGTGTAGAACCA320.4125838060856112No Hit
TACCTCAGTTTGCCCCCATGGGTCACTAAGATGTGGCAAGATGGCAAACT280.36101083032490977No Hit
GTTGGCGGATACCCTTGTGGAGGAGGAGGAAGATGTGGCAAGATGGCAAA280.36101083032490977No Hit
GTTGGCGGATACCCTTCCCAGAGATGTGGCAAGATGGCAAACTGGTGTAG270.3481175863847344No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG270.3481175863847344No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCATGTACTCTGCGTTGA250.3223310985043837No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA240.30943785456420836No Hit
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCCATGTACTCTGCGTTG240.30943785456420836No Hit
GTTGGCGGATACCCTTGGCAAGATGTGGCAAGATGGCAAACTGGTGTAGA230.296544610624033No Hit
GTTGGCGGATACCCTTGCTCTGTGTTGGCGGATACCCATGTACTCTGCGT220.28365136668385765No Hit
GTTGGCGGATACCCTTGCAAGATGTGGCAAGATGGCAAACTGGTGTAGAA210.2707581227436823No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC200.25786487880350695No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA190.2449716348633316No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA190.2449716348633316No Hit
TACCTCAGTTTGCCCCCATGAGTGAGGAGGCAGAAGATGTGGCAAGATGG180.23207839092315627No Hit
TACCTCAGTTTGCCCCCATGGCACAGATGTGGCAAGATGGCAAACTGGTG170.21918514698298092No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA160.2062919030428056No Hit
GTTGGCGGATACCCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC160.2062919030428056Clontech SMART CDS Primer II A (96% over 26bp)
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA150.19339865910263024No Hit
GTTGGCGGATACCCTTCCCTACATTGTTCCAACAGATGTGGCAAGATGGC140.18050541516245489No Hit
GTTGGCGGATACCCTTCACAAAGAAAAGAGAACTCTCCCCGGGGCTCCCG140.18050541516245489No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCAAACTGGTGTAGAACCAT140.18050541516245489No Hit
GTTGGCGGATACCCTTCCGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT130.16761217122227953No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA130.16761217122227953No Hit
GTTGGCGGATACCCTTCCCTAAGATGTGGCAAGATGGCAAACTGGTGTAG120.15471892728210418No Hit
GTTGGCGGATACCCTTGGACGTAGATGTGGCAAGATGGCAAACTGGTGTA120.15471892728210418No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT120.15471892728210418No Hit
GTTGGCGGATACCCTTCCAACTTCTAAGATGTGGCAAGATGGCAAACTGG120.15471892728210418No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG110.14182568334192883No Hit
GTTGGCGGATACCCTTCCATAAGTCAAGATGTGGCAAGATGGCAAACTGG110.14182568334192883No Hit
TACCTCAGTTTGCCCCCATGCACCATAAGATGTCCTCAACTGGTGTAGAA110.14182568334192883No Hit
GTTGGCGGATACCCCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT100.12893243940175347Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTGCTGTGTTGGCGGATACCCATGTACTCTGCGTTG100.12893243940175347No Hit
GTTGGCGGATACCCTTCCCTCAGTCCCCAAAGTCCAATGTATCATTCTAA100.12893243940175347No Hit
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT100.12893243940175347No Hit
GTTGGCGGATACCCTTCCAGAAGTCGAGATGTGGCAAGATGGCAAACTGG100.12893243940175347No Hit
GTTGGCGGATACCCTTCCATATGAATGAATTCAAAAGAGCTTGTAAAAAC100.12893243940175347No Hit
GTTGGCGGATACCCTTCCTGTGTTGGCGGATACCCTTCCTGTGTTGGCGG90.11603919546157813No Hit
GTTGGCGGATACCCTTCTAAGATGTGGCAAGATGGCAAACTGGTGTAGAA90.11603919546157813No Hit
GTTGGCGGATACCCTTCCCAAAGATGTGGCAAGATGGCAAACTGGTGTAG90.11603919546157813No Hit
GTTGGCGGATACCCTTCCCTATAATACATACAAAACAGTTTAAGAATTAA90.11603919546157813No Hit
GTTGGCGGATACCCTTGCCTGTGTTGGCGGATACCCATGTACTCTGCGTT90.11603919546157813No Hit
GTTGGCGGATACCCTTCCATGTTGGCGGATACCCTTCTGTGTTGGCGGAT90.11603919546157813No Hit
GTTGGCGGATACCCTTCCAGACCGCCAGCTAAGGTCCCAAAGTCATGGTT80.1031459515214028No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATAC80.1031459515214028No Hit
GTTGGCGGATACCCTTCCATCACTTCCACGCAAGATGTGGCAAGATGGCA80.1031459515214028No Hit
GTTGGCGGATACCCTTGCTCCCATGTACTCTGCGTTGATACCACTGCTTC80.1031459515214028Clontech SMART CDS Primer II A (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)