FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8388
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length138-145
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT345741.2136385312351No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT125514.961850262279446No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC84610.085836909871244No Hit
TGCCATCTTGCCACATCTTCCTTAGCATTCTGTTCTTTGAAATGGAAGGG5856.974248927038626No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA3864.601812112541726No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1782.1220791607057703No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1491.776347162613257No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA1141.3590844062947067No Hit
TGCCATCTTGCCACATCTTAGGAAGCATTCACTACTCCCGTGTGCCCCGC770.9179780639008107No Hit
TGCCATCTTGCCACATCTTGTGCCATCAAGGGTATCCGCCAACTTGAGGA340.40534096328087743No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG210.2503576537911302No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC160.19074868860276584No Hit
GAGGACATCTTGCCACATCTTCCTTAGCATTCTGTTCTTTGAAATGGAAG140.16690510252742013No Hit
TGCCATCTTGCCACATCTACTCTGCGTTGATACCACTGCTTCCCATGTAC140.16690510252742013Clontech Universal Primer Mix Long (96% over 26bp)
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT120.14306151645207438No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG110.13113972341440153No Hit
TGCCATCTTGCCACATCTTGTTCAGTGGAGATCCAAGATTGGGGCTGAGG110.13113972341440153No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT110.13113972341440153No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT110.13113972341440153No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT100.11921793037672865No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC90.1072961373390558No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT90.1072961373390558No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTCCCCAGT90.1072961373390558No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT90.1072961373390558No Hit
TTCTGAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT90.1072961373390558No Hit
GAGGACATCTTATGGTGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCT90.1072961373390558No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTCTCCCAGT90.1072961373390558No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)