FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8388
Total Bases1.2 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA326538.92465426800191No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG192322.925608011444922No Hit
GTTGGCGGATACCCTTCCATTTCAAAGAACAGAATGCTAAGGAAGATGTG5136.115879828326181No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC3714.422985216976634No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1802.1459227467811157No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1481.764425369575584No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC1071.2756318550309966No Hit
GTTGGCGGATACCCTTCCAGTAGAAGCGGGGCACACGGGAGTAGTGAATG790.9418216499761564No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA500.5960896518836434No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT460.5484024797329518No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA420.5007153075822603No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA350.41726275631855037No Hit
GTTGGCGGATACCCTTGATGGCACAAGATGTGGCAAGATGGCAAACTGGT340.40534096328087743No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC280.33381020505484027Clontech Universal Primer Mix Long (96% over 26bp)
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG280.33381020505484027No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG270.3218884120171674No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG260.3099666189794945No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG250.2980448259418217No Hit
TACCTCAGTTTGCCCCCCCGAAGCAGTGGTATCAACGCAGAGTACATGGG230.2742012398664759Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA220.26227944682880305No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA180.2145922746781116No Hit
GTTGCGGATACCCTTCCATTTCAAAGAACAGAATGCTAAGGAAGATGTGG180.2145922746781116No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA170.20267048164043872No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA170.20267048164043872No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG160.19074868860276584No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA150.17882689556509299No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG140.16690510252742013No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG130.15498330948974726No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG120.14306151645207438No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC110.13113972341440153No Hit
GTGGCGGATACCCTTCCATTTCAAAGAACAGAATGCTAAGGAAGATGTGG110.13113972341440153No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA110.13113972341440153No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG100.11921793037672865No Hit
TACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATACTCCTTCA100.11921793037672865No Hit
GTTGGCGGATACCCTTCATTAAGTGCAGCAAAATTAATATGCCCTAACAA100.11921793037672865No Hit
GTTGGCGATACCCTTCCATTTCAAAGAACAGAATGCTAAGGAAGATGTGG100.11921793037672865No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA90.1072961373390558No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG90.1072961373390558No Hit
TACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATACTCCTTCA90.1072961373390558No Hit
GTTGGCGGATACCCATGTCCCTTACACACACGCAAAATACTCCTTCAGCG90.1072961373390558No Hit
TACCTCAGTTTGCCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGT90.1072961373390558No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA90.1072961373390558No Hit
GTTGGCGGTACCCTTCCATTTCAAAGAACAGAATGCTAAGGAAGATGTGG90.1072961373390558No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)