FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33028
Total Bases4.7 Mbp
Sequences flagged as poor quality0
Sequence length35-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA2077262.892091558677485No Hit
GTTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGG27308.265713939687538No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC20266.134189172823059No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG9102.755237979895846No Hit
GTTGGCGGATACCCTTCCCATTTCAGTGCACCATAAGATGTCCTCAACTG3481.053651447256873No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2760.8356545961002786No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1900.5752694683299019No Hit
TACCTCAGTTTGCCCCCATGTACTCTGGGGAAGCAGTGGTATCAACGCAG1790.5419643938476444No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1720.5207702555407533No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1520.4602155746639215No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1410.42691050018166404No Hit
GTTGGCGGATACCCTTTCCATTTCAGTGCACCATAAGATGTCCTCAACTG1060.32093980864720845No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG940.2846070001211094No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG900.272496063945743No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG890.2694683299019014No Hit
GTTGGCGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT800.24221872350732712No Hit
GTTGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT750.22708005328811917No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG750.22708005328811917No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG730.221024585200436No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT620.1877195107181785No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG550.16652537241128737No Hit
TACCTCAGTTTGCCCCCCCTGTGAAGCAGTGGTATCAACGCAGAGTACAT550.16652537241128737Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA520.15744217027976262No Hit
TACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATACTCCTTCA520.15744217027976262No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG500.15138670219207945No Hit
TACCTCAGTTTGCCCCCATGTCCCTCACACACACGCAAAATACTCCTTCA450.1362480319728715No Hit
TACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATACTCCTTCA440.13322029792902992No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATACTCCTTCA420.12716482984134672No Hit
TACCTCAGTTTGCCCCCATGGATGATGGCACAGGGGGGAAGCAGTGGTAT400.12110936175366356No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAACACTCCTTCA370.1120261596221388No Hit
TACCTCAGTTTGCCCCCATGTACTCTGCAGTGGTATCAACGCAGAGTACA370.1120261596221388Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTTGGCGGTACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT350.10597069153445561No Hit
TACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATACTCCTTCA350.10597069153445561No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACGCACACGCAAAATACTCCTTCA340.10294295749061402No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATGCTCCTTCA340.10294295749061402No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATATTCCTTCA340.10294295749061402No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)