FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4859
Total Bases704.4 kbp
Sequences flagged as poor quality0
Sequence length142-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT296461.00020580366331No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC4228.684914591479728No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACTTGAGG4218.664334225149208No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1002.058036633052068No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACTTGAG711.4612060094669685No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG681.3994649104754064No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA480.9878575838649928No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCGCCAACTTGAGGA170.3498662276188516No Hit
TGCCATCTTGCCACATCTGAAGAGGAAGGGTATCCGCCAACTTGAGGAGA140.28812512862728956No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT120.2469643959662482No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATTTGGTTTCCCAGTC120.2469643959662482No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT120.2469643959662482No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG120.2469643959662482No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATCCGCCAACTTGAG120.2469643959662482No Hit
TGCCATCTTGCCACATCTTCCTCCCCAAGCAGTGGTATCAACGCAGAGTA110.2263840296357275Clontech Universal Primer Mix Long (96% over 25bp)
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCAACTTGAGGA110.2263840296357275No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACACAGAA100.20580366330520683No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAATTGAGGA100.20580366330520683No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT90.18522329697468615No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTACCGCCAACTTGAGGA90.18522329697468615No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTGTTTGGTTTCCCAGT80.16464293064416546No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCCCAACTTGAGGA80.16464293064416546No Hit
TGCCATCTTGCCACATCTTATTTAACAACTATTAAAGCAATCATCTTTGG80.16464293064416546No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT80.16464293064416546No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG80.16464293064416546No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTCGGTTTCCCAGT70.14406256431364478No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGCTTCCCAGT70.14406256431364478No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT60.1234821979831241No Hit
GAGGACATCTTATGGTGCACTGGAATGGAAAGGGTATTTGGTTTCCCAGT60.1234821979831241No Hit
TGCCGTCTAACCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC60.1234821979831241No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGATCCGCCAACTTGAGGA60.1234821979831241No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT60.1234821979831241No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT60.1234821979831241No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGAGTATTTGGTTTCCCAGT50.10290183165260341No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTCCCCAGT50.10290183165260341No Hit
GAGGACATCTTATGGTGCATTGAAATGGAAAGGGTATTTGGTTTCCCAGT50.10290183165260341No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAAT50.10290183165260341No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)