FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4859
Total Bases698.7 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA282158.057213418398845No Hit
GTTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGG4098.41736982918296No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG3346.873842354393908No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC911.8728133360773822No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAAACAAAAGATTCG701.440625643136448No Hit
GTTGGCGGATACCCTTCCCATTTCAGTGCACCATAAGATGTCCTCAACTG691.420045276805927No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC661.358304177814365No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC470.9672772175344722No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG280.5762502572545791No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG220.452768059271455No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG210.43218769294093434No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG170.3498662276188516No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG160.3292858612883309No Hit
GTTGGCGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT160.3292858612883309No Hit
GTTGGCGGATACCCTTCCTCTTCAGATGTGGCAAGATGGCAAACTGGTGT140.28812512862728956No Hit
GTTGGCGGATACCCTTTCCATTTCAGTGCACCATAAGATGTCCTCAACTG120.2469643959662482No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA110.2263840296357275No Hit
GTTGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT110.2263840296357275No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA100.20580366330520683No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG90.18522329697468615No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT90.18522329697468615No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA90.18522329697468615No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA90.18522329697468615No Hit
GTTGGCGGTACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT90.18522329697468615No Hit
TTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT80.16464293064416546No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG80.16464293064416546No Hit
GTTGGGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT80.16464293064416546No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG80.16464293064416546No Hit
GTTGGCGGATACCCTTCCATGAAGGCTTTAACACAGAATCAAAAGATTCG70.14406256431364478No Hit
GTTGGCGGATACCCTTCCATTCACCAAAGATGATTGCTTTAATAGTTGTT70.14406256431364478No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA70.14406256431364478No Hit
TACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATACTCCTTCA70.14406256431364478No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG70.14406256431364478No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA60.1234821979831241No Hit
GTTGGCGGATCCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT60.1234821979831241No Hit
TACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATACTCCTTCA60.1234821979831241No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATATTCCTTCA60.1234821979831241No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACATGCAAAATACTCCTTCA60.1234821979831241No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA50.10290183165260341No Hit
TACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA50.10290183165260341No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATGCTCCTTCA50.10290183165260341No Hit
TACCTCAGTTTACCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA50.10290183165260341No Hit
TACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATACTCCTTCA50.10290183165260341No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG50.10290183165260341No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)