FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29478
Total Bases4.2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA1765459.888730578736684No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG489516.605604179388017No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC12844.355790759210258No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC4751.611371192075446No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC3381.1466178166768437No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC2930.9939615984802225No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1640.556347106316575No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1180.4002985277155845No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1150.39012144650247643No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1140.3867290860981071No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1110.37655200488499896No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1100.3731596444806296No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG770.26121175113644074No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA740.2510346699233327No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA590.20014926385779225No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG580.1967569034534229No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG580.1967569034534229No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG580.1967569034534229No Hit
GTTGGCGGATACCCTTCCTCTAAGATGTGGCAAGATGGCAAACTGGTGTA550.1865798222403148No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA520.1764027410272067No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA500.169618020218468No Hit
TACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATACTCCTTCA470.1594409390053599No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA430.1458714973878825No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATACTCCTTCA420.14247913698351314No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA410.13908677657914376No Hit
TACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATACTCCTTCA410.13908677657914376No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG390.13230205577040505No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAACACTCCTTCA380.1289096953660357No Hit
GTTGGCGGATACCCATGTCCCTTACACACACGCAAAATACTCCTTCAGCG380.1289096953660357No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACGCACGCAAAATACTCCTTCA370.12551733496166634No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA370.12551733496166634No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATATTCCTTCA370.12551733496166634No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG360.12212497455729697No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA330.11194789334418888No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG320.10855553293981954No Hit
TACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATACTCCTTCA310.10516317253545017No Hit
GTTGGCGGATACCCTTCCTTTATCCCAACAGATGTGGCAAGATGGCAAAC300.10177081213108082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)