FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45043
Total Bases6.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1192526.4747019514686No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA1148425.495637501942586No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC879019.51468596674289No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA26345.847745487645139No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT13743.0504184889994006No Hit
TGCCATCTTGCCACATCTGACAAATTGAAAACAGCCTGCAACCTCTCTGA6381.416424305663477No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG5991.3298403747530136No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG4911.0900694891548075No Hit
TGCCATCTTGCCACATCTTACAAATTGAAAACAGCCTGCAACCTCTCTGA2690.5972071132029394No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT2430.5394844925959639No Hit
TGCCATCTTGCCACATCTCTGCCCATTAATTGGAGACTTTACAACATTTA1450.32191461492351753No Hit
TGCCATCTTGCCACATCTTAGCCGGGCGCGACCCGCTCCGGGGACAGTGC1170.25975179273139No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG1160.25753169193881403No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT1050.2331105832204782No Hit
TGCCATCTTGCCACATCTTCCCCAGGCTGCAGGTACTCCTCACTTGGTAT1040.23089048242790222No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT960.21312967608729436No Hit
TGCCATCTTGCCACATCTTGGCCGGGCGCGACCCGCTCCGGGGACAGTGC940.2086894745021424No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT890.19758897053926247No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC780.17316786182092667No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG690.15318695468774282No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT610.13542614834713496No Hit
TGCCATCTTGCCACATCTGCTAACCTTAGCAAATGACAGGGTTCCTAACG560.12432564438425504No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT550.12210554359167906No Hit
TGCCATCTTGCCACATCTTATCTGAGGTCGCGAAGGGTATCCGCCAACTT460.10212463645849522No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)