FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7110
Total Bases1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA265037.27144866385373No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC155121.814345991561183No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACTTGAGG3765.288326300984529No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT1882.6441631504922647No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1572.208157524613221No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1502.109704641350211No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG1041.4627285513361463No Hit
TGCCATCTTGCCACATCTTCCTCCCCAAGCAGTGGTATCAACGCAGAGTA971.3642756680731365Clontech Universal Primer Mix Long (96% over 25bp)
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACTTGAG680.9563994374120955No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACACAGAA290.4078762306610408No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT270.37974683544303794No Hit
TGCCGTCTAACCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC270.37974683544303794No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT240.33755274261603374No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCGCCAACTTGAGGA210.29535864978902954No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC210.29535864978902954No Hit
TGCCATCTTGCCACATCTTCCTCCCTAAGCAGTGGTATCAACGCAGAGTA180.25316455696202533Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGCCATCTTGCCACATCTTAGAAGCTGACTCCTTTCCCCATCTTCCCATC180.25316455696202533No Hit
GAGGACATCTTATGGTGCACTGAAAGAAGTTGTTGCACATATAGTGAAGG160.2250351617440225No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG160.2250351617440225No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTACCGCCAACTTGAGGA150.21097046413502107No Hit
TGCCATCTTGCCACATCTTGTGCCATCAAGGGTATCCGCCAACTTGAGGA150.21097046413502107No Hit
TGCCATCTTGCCACATCTGCCCAAGGAACTTTTTTATTGAGGTATTCTAA140.19690576652601968No Hit
TGCCATCTTGCCACATCTTATCTGAGGTCGCGAAGGGTATCCGCCAACTT140.19690576652601968No Hit
TGCCATCTTGCCACATCTGCACCGCTGCGGTGAGCCTTGAAGCCTAGGGC130.1828410689170183No Hit
TGCCATCTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT130.1828410689170183No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCAACTTGAGGA120.16877637130801687No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACACAGGA120.16877637130801687No Hit
TGCCATCTTGCCACATCTTCTGCCATGGGAAGCAGTGGTATCAACGCAGA110.15471167369901548Clontech Universal Primer Mix Long (100% over 21bp)
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT110.15471167369901548No Hit
TGCCATCTTGCCACATCTTAGAGTACGGTCAAGCAGTGGTATCAACGCAG110.15471167369901548Clontech Universal Primer Mix Long (100% over 21bp)
TGCCTCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT100.14064697609001406No Hit
TGCCATTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT90.12658227848101267No Hit
TGCCATCTTGCCACATCTTATCTCCGCCATTGTAGTGCCAAAGCAGTGGT90.12658227848101267No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT90.12658227848101267No Hit
TGCCATCTTGCCACATCTTGGAAGGGTATCCGCCAACTTGAGGAGACCAA80.11251758087201125No Hit
TGCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT80.11251758087201125No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCCCAACTTGAGGA80.11251758087201125No Hit
GAGGACATCTTATGGTGCACTGAAAACTATGAAACACTGATGAAAGAAAG80.11251758087201125No Hit
GAGGACATCTTATGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC80.11251758087201125No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGCTTCCCAGTCCACTA80.11251758087201125No Hit
TGCCATCTTGCCACATCTTAGCAGGGTATCCGCCAACTTGAGGAGACCAA80.11251758087201125No Hit
TGCCATCTTGCCACATCTTCCTCCCAAAGCAGTGGTATCAACGCAGAGTA80.11251758087201125Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTCCCCAGTCCACTA80.11251758087201125No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)