FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27603
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA1348848.86425388544723No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG581521.066550737238707No Hit
GTTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGG15685.6805419700757165No Hit
GTTGGCGGATACCCTTCCACACACGCAAAATACTCCTTCAGCGGAGCGAA7332.6555084592254463No Hit
GTTGGCGGATACCCTTCCATACACGCAAAATACTCCTTCAGCGGAGCGAA3271.1846538419736985No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC2730.9890229322899685No Hit
GTTGGCGGATACCCTTCCCATTTCAGTGCACCATAAGATGTCCTCAACTG2500.9056986559431947No Hit
GTTGGCGGATACCCTTCCATTCACCAAAGATGATTGCTTTAATAGTTGTT2450.8875846828243307No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2360.8549795312103756No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1670.605006702170054No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1350.48907727420932506No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1250.4528493279715973No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1170.42386697098141507No Hit
GTTGGCGGATACCCTTACACACACGCAAAATACTCCTTCAGCGGAGCGAA860.31156033764445895No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG840.30431474839691336No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA820.2970691591493678No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG790.2862007752780495No Hit
GTTGGCGGATACCCTTTCCATTTCAGTGCACCATAAGATGTCCTCAACTG790.2862007752780495No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG700.2535956236640945No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA680.2463500344165489No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA680.2463500344165489No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG670.24272723979277613No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA650.2354816505452306No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG560.2028764989312756No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG540.19563090968373No Hit
TACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATACTCCTTCA420.15215737419845668No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG400.14491178495091112No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA400.14491178495091112No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA390.14128899032713835No Hit
GTTGGCGGATACCCTTGAGCACACCAGACTTGCAGAAAAAGCATACTCCA340.12317501720827445No Hit
GTTGGCGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGGT330.11955222258450168No Hit
TACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATACTCCTTCA320.11592942796072891No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACGCACACGCAAAATACTCCTTCA300.10868383871318335No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA290.10506104408941057No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG280.1014382494656378No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT280.1014382494656378No Hit
GTTGGCGGATACCCTTGCACACACGCAAAATACTCCTTCAGCGGAGCGAA280.1014382494656378No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)