FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44542
Total Bases6.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT2807463.02815320371784No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC481010.798796641372189No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA36828.266355350006735No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACTTGAGG17033.8233577297831256No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT7061.5850208791702216No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG4260.9564007004624848No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG3000.6735216200440034No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACTTGAG2840.63760046697499No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATCCGCCAACTTGAG1340.3008396569529882No Hit
TGCCATCTTGCCACATCTTATTTAACAACTATTAAAGCAATCATCTTTGG1100.24695792734946792No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT800.17960576534506756No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATTTGGTTTCCCAGTC570.12796910780836065No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT570.12796910780836065No Hit
GAGGACATCTTATGGTGTACTGAAATGGAAAGGGTATTTGGTTTCCCAGT550.12347896367473396No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCAACTTGAGGA530.11898881954110728No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG530.11898881954110728No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT520.11674374747429393No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGCTTCCCAGT520.11674374747429393No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT510.11449867540748059No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT500.11225360334066724No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT470.10551838714022721No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)