FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3500
Total Bases507.3 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT126736.199999999999996No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC88825.371428571428574No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA1514.314285714285714No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1333.8No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT1143.257142857142857No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG832.3714285714285714No Hit
TGCCATCTTGCCACATCTTTTGGACACCTAACTGTCAGGAGTTGTGGAAG631.7999999999999998No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG511.4571428571428573No Hit
TGCCATCTTGCCACATCTTATTTAACAACTATTAAAGCAATCATCTTTGG351.0No Hit
TGCCATCTTGCCACATCTTTTGGACACCTAACTGTCAGGAGTTATGGAAG351.0No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT240.6857142857142857No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA230.6571428571428571No Hit
TGCCATCTTGCCACATCTTAGCAGATAAGTGGTCTGGTTTGCATAAACAT190.5428571428571428No Hit
TGCCATCTTGCCACATCTTTGCAGATAAGTGGTCTGGTTTGCATAAACAT180.5142857142857142No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT150.4285714285714286No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC130.37142857142857144No Hit
TGCCGTCTAACCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC120.34285714285714286No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG100.2857142857142857No Hit
TGCCATCTTGCCACATCTACTGGCGCGAGGAAGGGTATCCGCCAACTTGA100.2857142857142857No Hit
TGCCATCTTGCCACATCTTCGCACCTGACAATGTCCTCCGAAGGGTATCC90.2571428571428571No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT90.2571428571428571No Hit
TGCCATCTTGCCACATCTTATCTGAGGTCGCGAAGGGTATCCGCCAACTT90.2571428571428571No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG80.2285714285714286No Hit
TGCCATCTTGCCACATCTTCGTAACTTCGGGAGAAGGGTATCCGCCAACT80.2285714285714286No Hit
TGCCATCTTGCCACATCTCCGTAACTTCGGGAGAAGGGTATCCGCCAACT70.2No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT60.17142857142857143No Hit
TGCCATCTTGCCACATCTTCAGTCTGCCAGCCCCTCCACTTTGCAGAAGC60.17142857142857143No Hit
GAGGACATCTTATGGTGTACTGAAATGGAAAGGGTATTTGGTTTCCCAGT60.17142857142857143No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT50.14285714285714285No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTCCCCAGT50.14285714285714285No Hit
TGCCATCTTGCCACATCTTAGAAGTTGGGGGACGCCGACCGCTCGGGGGT50.14285714285714285No Hit
TGCCATCTTGCCACATCTGATCCCGCAGCTCTAAAGGGTATCCGCCAACT50.14285714285714285No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT50.14285714285714285No Hit
GAGGACATCTTATGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC50.14285714285714285No Hit
GAGGACATCTTATGGTGCATGGGGGCAAACTGAGGTATTGAGGAGACCAA40.1142857142857143No Hit
TGCCATCTTGCCACATCTTAGAAGCTTAGTTGAAATCATTGAACATGGTC40.1142857142857143No Hit
TGCCATTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT40.1142857142857143No Hit
TGCCATCTTGCCACATCTTCCATGATATATTATAATGACCTATTTGTGCG40.1142857142857143No Hit
GAGGACATCTTATGGTGCACTGAAGCAGTGGTATCAACGCAGAGTACATG40.1142857142857143Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTCCGCACCTGACAATGTCCTCCGAAGGGTATCC40.1142857142857143No Hit
TGCCATCTTGCCACATCTCCTTCTACCCTTGGCTCAGAGCAAAGTTGGGC40.1142857142857143No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCAAGT40.1142857142857143No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)