FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32743
Total Bases4.7 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT2170966.30119414836759No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC485914.839813089820725No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA12743.890907980331674No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG3951.206364719176618No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT3771.1513911370369239No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG3681.123904345967077No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA2930.894847753718352No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG1040.3176251412515652No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT540.16492074641908194No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT500.15270439483248327No Hit
TGCCATCTTGCCACATCTTACTAGGACGCCAAAAAAGGGGGGGCAAACTG500.15270439483248327No Hit
TGCCATCTTGCCACATCTTACTAGGACGCCAAAAAGGGGGGGCAAACTGA490.14965030693583362No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT490.14965030693583362No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGCTTCCCAGT480.14659621903918396No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT470.14354213114253428No Hit
GAGGACATCTTATGGTGTACTGAAATGGAAAGGGTATTTGGTTTCCCAGT430.1313257795559356No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCTCAGT430.1313257795559356No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGC410.1252176037626363No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC410.1252176037626363No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAAT400.12216351586598663No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT400.12216351586598663No Hit
TGCCATCTTGCCACATCTACTCTAATGTATGTATATAAAATCATTTCCCC380.11605534007268728No Hit
TGCCATCTTGCCACATCTTACTAGGACGCCAAAAAAGGGGGGAGGGGGCA370.11300125217603764No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGATATTTGGTTTCCCAGT360.10994716427938796No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT360.10994716427938796No Hit
GAGGACATCTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGTC340.10383898848608862No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)