FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32743
Total Bases4.7 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA2004761.225300064135844No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG475714.528296124362457No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC12233.7351494976025412No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC3921.197202455486669No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC3641.1116879943804783No Hit
GTTGGCGGATACCCTTCCACACACGCAAAATACTCCTTCAGCGGAGCGAA2790.8520905231652567No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC2750.8398741715786581No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1780.5436276456036405No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1400.42757230553095316No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1350.4123018660477049No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1270.3878691628745075No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1110.3390037565281129No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1100.33594966863146325No Hit
GTTGGCGGATACCCTTCCATAAAGGGTTTAACACAGAATCAAAAGATTCG1060.3237333170448645No Hit
GTTGGCGGATACCCTTCCATACACGCAAAATACTCCTTCAGCGGAGCGAA920.28097608649176925No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA870.2657056470085209No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG850.2595974712152216No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG690.2107320648688269No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG680.20767797697217724No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG670.20462388907552762No Hit
GTTGGCGGATACCCTTACACACACGCAAAATACTCCTTCAGCGGAGCGAA620.18935344959227926No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG550.16797483431573162No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA540.16492074641908194No Hit
TACCTCAGTTTGCCCCCCCTTTTTGGCGTCCTAGTAAGATGTGGCAAGAT510.15575848272913295No Hit
TACCTCAGTTTGCCCCCCCTTTTTTGGCGTCCTAGTAAGATGTGGCAAGA510.15575848272913295No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG470.14354213114253428No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA470.14354213114253428No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA470.14354213114253428No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA460.14048804324588463No Hit
TACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATACTCCTTCA460.14048804324588463No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA450.13743395534923494No Hit
TACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA420.12827169165928595No Hit
GTTGGCGGATACCCATGTCCCTTACACACACGCAAAATACTCCTTCAGCG420.12827169165928595No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATACTCCTTCA410.1252176037626363No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG400.12216351586598663No Hit
TACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATACTCCTTCA380.11605534007268728No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA370.11300125217603764No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAACACTCCTTCA360.10994716427938796No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAGTACTCCTTCA360.10994716427938796No Hit
TACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATACTCCTTCA350.10689307638273829No Hit
GTTGGCGGATACCCTTCCAGAAGAATACAGAGATTATTTTATTCGAAATT350.10689307638273829No Hit
TACCTCAGTTTGCCCCCTTTCCCCCTTTTTTGGCGTCCTAGTAAGATGTG330.10078490058943897No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)