FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4564
Total Bases661.5 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA205044.916739702015775No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA134629.491673970201575No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT681.4899211218229622No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACTTGAGG410.8983347940403156No Hit
TGCCATCTTGCCACATCTTCCTCCCCAAGCAGTGGTATCAACGCAGAGTA400.8764241893076249Clontech Universal Primer Mix Long (96% over 25bp)
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG220.48203330411919365No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG220.48203330411919365No Hit
TGCCATCTTGCCACATCTTCCTTCAGCAAGCAGTGGTATCAACGCAGAGT200.43821209465381245Clontech Universal Primer Mix Long (100% over 25bp)
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT190.4163014899211218No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT180.3943908851884312No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT170.37248028045574055No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT140.3067484662576687No Hit
TGCCATCTTGCCACATCTTACTTCTTCAGAAAGCAGTGGTATCAACGCAG130.2848378615249781No Hit
TGCCATCTTGCCACATCTTAGAGTACGGTCAAGCAGTGGTATCAACGCAG130.2848378615249781Clontech Universal Primer Mix Long (100% over 21bp)
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT110.24101665205959683No Hit
AAGGTGGATGCCGCTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCTAC100.21910604732690622No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT100.21910604732690622No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT100.21910604732690622No Hit
TGCCCTCTCTGCCTTTTCTCCCCAGGGTATTTGGTTTCCCAGTCCACTAT90.1971954425942156No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACTTGAG90.1971954425942156No Hit
AAGGTGGATGGTGAGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT90.1971954425942156No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT80.175284837861525No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGCCCACTA70.15337423312883436No Hit
TGCCATCTTGCCACATCTTCCTCCCTAAGCAGTGGTATCAACGCAGAGTA70.15337423312883436Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGCCATCTTGCCACATCTTCCTTAGCATTCTGTTCTTTGAAATGGAAGGG70.15337423312883436No Hit
TGCCCTCTCTGCCTTTTCTCAAGCAGTGGTATCAACGCAGAGTACATGGG70.15337423312883436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTCCCCAGTCCACTA70.15337423312883436No Hit
GAGGACATCTTGCCACATCTTCCTCCCCAAGCAGTGGTATCAACGCAGAG60.13146362839614373Clontech Universal Primer Mix Long (95% over 24bp)
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGG60.13146362839614373No Hit
TGCCATCTTGCCACATCTTATCTGAGGTCGCGAAGGGTATCCGCCAACTT60.13146362839614373No Hit
TGTCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA60.13146362839614373No Hit
TGCCATCTTGCCACATCTTAGGAAACAAAATCACATTTCATCGTGTCCCT50.10955302366345311No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAACCACCTGCTTCCGGGCTGA50.10955302366345311No Hit
TGCCATCTTGCCACATCTCGAGAGTGGAAGGGTATCCGCCAACTTGAGGA50.10955302366345311No Hit
TGCCATCTTGCCACATCTGCTAAATAACTAAAATTAGCAGGCAAGGGTAT50.10955302366345311No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT50.10955302366345311No Hit
TGCCATCTTGCCACATCTTAGTCAAGCAGTGGTATCAACGCAGAGTACAT50.10955302366345311Clontech Universal Primer Mix Long (96% over 26bp)
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGCTTCCCAGTCCACTA50.10955302366345311No Hit
TGCCATCTTGCCACATCTTCCCAGGAATAACTGGTCCCCTGCACTTATAA50.10955302366345311No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT50.10955302366345311No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)