FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4564
Total Bases661.3 kbp
Sequences flagged as poor quality0
Sequence length139-145
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC198343.448729184925504No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC126627.73882559158633No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG541.1831726555652935No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT531.161262050832603No Hit
GTTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCAACTGG410.8983347940403156No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT280.6134969325153374No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC220.48203330411919365No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC220.48203330411919365No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA170.37248028045574055No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC170.37248028045574055Clontech Universal Primer Mix Long (96% over 26bp)
CGAAGTAGACCACATCACCATCCACCTTAACTGGTGTAGAACCATGTCGT160.35056967572305No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT150.3286590709903593No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT140.3067484662576687No Hit
CGAAGTAGACCGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT110.24101665205959683No Hit
CGAAGTAGACCAGCGGCATCCCGGCCCCACCCTGGACCTTAGCTCAGCAC100.21910604732690622No Hit
CGAAGTAGACCAGCGGCATCCACCTTAACTGGTGTAGAACCATGTCGTCA100.21910604732690622No Hit
GTTGGCGGATACCCTTGCTCTGTGTTGGCGGATACCCATGTACTCTGCGT100.21910604732690622No Hit
CGAAGTAGCCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT90.1971954425942156No Hit
GTTGGCGGATACCCTTCCCATTTCAGTGCACCATAAGATGTCCTCAACTG80.175284837861525No Hit
CGAAGTGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT80.175284837861525No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT80.175284837861525No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC70.15337423312883436No Hit
GTTGGCGGATACCCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTG70.15337423312883436Clontech SMART CDS Primer II A (96% over 26bp)
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGGCCTTAGCTCAGCAC60.13146362839614373No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATAC60.13146362839614373No Hit
GTGTCGTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACT60.13146362839614373No Hit
GAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT60.13146362839614373No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATAAGATGTGGCAAGATGGCAAA60.13146362839614373No Hit
GTTGGCGGATACCCCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT60.13146362839614373Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTCCGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT60.13146362839614373No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA50.10955302366345311No Hit
GTTGGCGGATACCCTTCCACTCTCGAGATGTGGCAAGATGGCAAACTGGT50.10955302366345311No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC50.10955302366345311No Hit
GTTGGCGGATACCCTTCCATTTCAAAGAACAGAATGCTAAGGAAGATGTG50.10955302366345311No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACGCGCAAAATAC50.10955302366345311No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAACAC50.10955302366345311No Hit
CGAAGAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT50.10955302366345311No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)