FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29137
Total Bases4.2 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1806562.00020592373957No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC605520.78113738545492No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA5601.921954902701033No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAATGGTATTTGGTTTCCCAGT3241.1119881937055975No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG3061.0502110718330646No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA2931.0055942615917906No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2410.8271270206266945No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT2190.751621649449154No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG830.28486117307890313No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT770.264268799121392No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT480.16473899166008854No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGC470.1613069293338367No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT450.154442804681333No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT440.15101074235508116No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT430.14757868002882932No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT430.14757868002882932No Hit
TGCCATCTTGCCACATCTCTTGCTTCCTGGATAAATGTGGGTTCACTTGG410.14071455537632566No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGCTTCCCAGT400.13728249305007378No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT360.1235542437450664No Hit
TGCCATCTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT360.1235542437450664No Hit
TGCCATTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT350.12012218141881456No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTCCCCAGT320.10982599444005904No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGATATTTGGTTTCCCAGT300.10296186978755535No Hit
GAGGACATCTTATGGTGCACTGGAATGGAAAGGGTATTTGGTTTCCCAGT300.10296186978755535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)