FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3090
Total Bases447.8 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT164453.20388349514563No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC1775.728155339805825No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1534.951456310679612No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1404.53074433656958No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA1254.0453074433656955No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA1053.3980582524271843No Hit
TGCCATCTTGCCACATCTACACAAGGGTATCCGCCAACTTGAGGAGACCA541.7475728155339807No Hit
TGCCATCTTGCCACATCTTGAAAAGAACTTTGGAAAGAGAGTTAAACAGT521.6828478964401297No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG150.48543689320388345No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC130.4207119741100324No Hit
TGCCATCTTGCCACATCTAACAACTCACAGCCAGAGTGAGTATGCGACGG120.3883495145631068No Hit
TGCCATCTTGCCACATCTGCTCTTGCCCTTTCCTATCAACTCCACAGAAC110.3559870550161813No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG100.3236245954692557No Hit
GAGGACATCTTATGGTGCATGGGGGCAAACTGAGGTATTGAGGAGACCAA90.2912621359223301No Hit
TGCCATCTTGCCACATCTTAGGGGTCACTTCCCACGTTAACTCTGGGCCA80.2588996763754045No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT80.2588996763754045No Hit
GAGGACATCTTATGGTGTACTGAAATGGAAAGGGTATTTGGTTTCCCAGT80.2588996763754045No Hit
TGCCATCTTGCCACATCTTGTGAAGGGTATCCGCCAACTTGAGGAGACCA70.22653721682847897No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG70.22653721682847897No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT70.22653721682847897No Hit
TGCCATCTTGCCACATCTGCCCAGGTCTGCCGTTCCCTCTTTTCGTCAGA70.22653721682847897No Hit
TGCCATCTTGCCACATCTTGGGAAGGGTATCCGCCAACTTGAGGAGACCA70.22653721682847897No Hit
TGCCATCTTGCCACATCTGTTGTTTTGATTTGCATTTCTCTAATTATCAG70.22653721682847897No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT60.1941747572815534No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT60.1941747572815534No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGCTTCCCAGT60.1941747572815534No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGCATTTGGTTTCCCAGT40.12944983818770225No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGAGTATTTGGTTTCCCAGT40.12944983818770225No Hit
GAGGACATCTTATGATGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT40.12944983818770225No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACTT40.12944983818770225No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGC40.12944983818770225No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)