FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3090
Total Bases444.7 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA155850.420711974110034No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG1725.566343042071197No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1534.951456310679612No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC1404.53074433656958No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC1193.8511326860841426No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC963.1067961165048543No Hit
GTTGGCGGATACCCTTGTGTAGATGTGGCAAGATGGCAAACTGGTGTAGA541.7475728155339807No Hit
GTTGGCGGATACCCTTCCAACAATTTCACGTACTGTTTAACTCTCTTTCC401.2944983818770228No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG170.5501618122977346No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG160.517799352750809No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCAAACTG150.48543689320388345No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG130.4207119741100324No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG120.3883495145631068No Hit
GTTGGCGGATACCCTTGGAGGTTAATGCCATCAAGAAGGGTGAGTATGGG110.3559870550161813No Hit
TACCTCAGTTTGCCCCCATGCACCATAAGATGTCCTCAACTGGTGTAGAA90.2912621359223301No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG90.2912621359223301No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG80.2588996763754045No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT80.2588996763754045No Hit
GTTGGCGGATACCCTTCCTTTCTGTTGGGGAAGTCACACTGTGGTAGGGT80.2588996763754045No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA70.22653721682847897No Hit
GTTGGCGGATACCCTTCTGGCTGTTCTGTGGAGTTGATAGGAAAGGGCAA70.22653721682847897No Hit
GTTGGCGGATACCCTTCACAAGATGTGGCAAGATGGCAAACTGGTGTAGA70.22653721682847897No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCAAACTGGTGTAGAACCAT70.22653721682847897No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG70.22653721682847897No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG60.1941747572815534No Hit
GTTGGCGGATACCCTTCCCAAGATGTGGCAAGATGGCAAACTGGTGTAGA60.1941747572815534No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCAACTGGTGTAGAACCATGTC60.1941747572815534No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA60.1941747572815534No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA50.16181229773462785No Hit
TACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATACTCCTTCA50.16181229773462785No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA40.12944983818770225No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCAAA40.12944983818770225No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA40.12944983818770225No Hit
GTTGGCGGATACCCTTCCTTACACCTTATACAAAAATTAACTCAAGATGG40.12944983818770225No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA40.12944983818770225No Hit
GTTGGCGGATACCCTTCCGGCGGTTTGTTAAGTCAGATGTGGCAAGATGG40.12944983818770225No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATATTCCTTCA40.12944983818770225No Hit
TACCTCAGTTTGCCCCCATGTCCCATACACACACGCAAAATACTCCTTCA40.12944983818770225No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)