FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16584
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA906454.655089242643506No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC245814.821514712976363No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG7824.715388326097443No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC5553.346599131693198No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC3712.2370959961408587No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2991.8029425952725517No Hit
GTTGGCGGATACCCTTCCTTTCTCTGACCTTTTCTCTGTCTCTTCATCAG990.5969609261939218No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG700.4220935841775205No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG620.3738543174143753No Hit
TACCTCAGTTTGCCCCCATTTCCCTTACACACACGCAAAATACTCCTTCA530.31958514230583696No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG530.31958514230583696No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG510.30752532561505064No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG390.23516642547033287No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG350.21104679208876026No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG340.20501688374336707No Hit
GTTGGCGGATACCCTTCCTGGTCCTAAGGTTACAGGCAGCCTTGCTGTGA300.1808972503617945No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG280.1688374336710082No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG280.1688374336710082No Hit
GTTGGCGGATACCCTTCCAGAATTTTAGATATTAGAATTAAAATTACTGA270.16280752532561504No Hit
GTTGGCGGATACCCTTCCTCAGAGCAGCCACCACTCACTTGGGGCAAGAC260.1567776169802219No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTCG260.1567776169802219No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA260.1567776169802219No Hit
GTTGGCGGATACCCTTCCAAAGTCATGGTTAAGTGGGAAAGATGTGGCAA240.1447178002894356No Hit
TACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATACTCCTTCA240.1447178002894356No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG240.1447178002894356No Hit
TACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATACTCCTTCA240.1447178002894356No Hit
GTTGGCGGATACCCTTCCATTCTCTGACCTTTTCTCTGTCTCTTCATCAG230.13868789194404244No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA230.13868789194404244No Hit
TACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATACTCCTTCA220.1326579835986493No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT200.120598166907863No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA170.10250844187168354No Hit
GTTGGCGGATACCCATGTCCCTTACACACACGCAAAATACTCCTTCAGCG170.10250844187168354No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACATGCAAAATACTCCTTCA170.10250844187168354No Hit

[WARN]Adapter Content

Adapter graph