FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1538
Total Bases221.9 kbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA96762.8738621586476No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT1278.257477243172952No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG342.2106631989596877No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG291.8855656697009102No Hit
TGCCATCTTGCCACATCTTGTGCCATCAAGGGTATCCGCCAACTTGAGGA130.8452535760728219No Hit
TGCCATCTTGCCACATCTTAGGAGGGAAGGAAGGGTATCCGCCAACTTGA110.7152145643693107No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG60.3901170351105332No Hit
TGCCATCTTGCCACATCTGTTCTGTTGAAGATGGAAGGGTATCCGCCAAC60.3901170351105332No Hit
TGCCATCTTGCCACATCTAGGGAGGGAAGGAAGGGTATCCGCCAACTTGA60.3901170351105332No Hit
TGCCATCTTGCCACATCTTGGGAGGGAAGGAAGGGTATCCGCCAACTTGA60.3901170351105332No Hit
TGCCCTCTCTGCCTTTTCTCCCCAGGGTATTTGGTTTCCCAGTCCACTAT50.3250975292587776No Hit
TGCCATCTTGCCACATCTTTCTCTCTTCCTCTTCAAGGGTATCCGCCAAC50.3250975292587776No Hit
TGCCATCTTGCCACATCTTAGGCCAAGCAGTGGTATCAACGCAGAGTACA50.3250975292587776Clontech Universal Primer Mix Long (96% over 29bp)
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT50.3250975292587776No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGTA40.26007802340702213Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTGGAAGGGGAAGGGTATCCGCCAACTTGAGGA40.26007802340702213No Hit
TGCCATCTTGCCACATCTTCTTCTGCGCCTTGGTCAAGGGTATCCGCCAA40.26007802340702213No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTACTTGGTTTCCCAGTCCACTA40.26007802340702213No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT40.26007802340702213No Hit
TGCCATCTTGCCACATCTTCTTCCACTCCCTCTCAAGGGTATCCGCCAAC40.26007802340702213No Hit
TGCCATCTTGCCACATCTGTGCCATCTTGCAAGGGTATCCGCCAACTTGA40.26007802340702213No Hit
TGCCATCTTGCCACATCTAGGGAGGGATGGAAGGGTATCCGCCAACTTGA40.26007802340702213No Hit
TGCCCTCTCTGCCTTTTCTCCCCCGGGGTATTTGGTTTCCCAGTCCACTA40.26007802340702213No Hit
TGCCATCTTGCCACATCTGCTGGCCAAGGGTATCCGCCAACTTGAGGAGA40.26007802340702213No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATCTGGTTTCCCAGTCCACTA30.1950585175552666No Hit
TGCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT30.1950585175552666No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTG30.1950585175552666No Hit
TGCCATCTTGCCACATCTTCGCCCAAGCAGTGGTATCAACGCAGAGTACA30.1950585175552666Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTCAAGCCAAGCAGTGGTATCAACGCAGAGTACA30.1950585175552666Clontech Universal Primer Mix Long (96% over 26bp)
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTAGTTTCCCAGTCCACTA30.1950585175552666No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA30.1950585175552666No Hit
GAGGACATCTTATGGTGCACTGAAGCAGTGGTATCAACGCAGAGTACATG30.1950585175552666Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTCGGAAGGGGAAGGGTATCCGCCAACTTGAGGA30.1950585175552666No Hit
TGCCATCTTGCCACATCTTAAGCCAAGCAGTGGTATCAACGCAGAGTACA30.1950585175552666Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTAGGAGGGAAGGAAGGGTATCCGCCAACACAG30.1950585175552666No Hit
TGCCATCTTGCCACATCTAGGGTATCCGCCAACTTGAGGAGACCAAGTCT30.1950585175552666No Hit
TGCCATCTTGCCACATCTTATGCCATCAAGGGTATCCGCCAACTTGAGGA20.13003901170351106No Hit
TGCCATCTTGCCACATCTTAGCCTCCCAAGGGTATCCGCCAACTTGAGGA20.13003901170351106No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGCGTATTTGGTTTCCCAGTCCACTA20.13003901170351106No Hit
TGCCATCTTGCCACATCTTAGAAGGGGAAGGGTATCCGCCAACTTGAGGA20.13003901170351106No Hit
TGCCATCTTGCCACATCTCTCAGGACCGACTGACCCATGTTCAACTGCTG20.13003901170351106No Hit
TGCCGTCTAACCACATCTTGTGCCATCAAGGGTATCCGCCAACTTGAGGA20.13003901170351106No Hit
TGCCATCTTGCCACATCTTACTCTTGCTTCTTGGAATAACTGCCCAAATA20.13003901170351106No Hit
TGCCCTCTCTGTCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA20.13003901170351106No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGCTTCCCAGTCCACTA20.13003901170351106No Hit
TGCCATCTTGCCACATCTCAAGCAAAGCAGTGGTATCAACGCAGAGTACA20.13003901170351106Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTCGGTTTCCCAGTCCACTA20.13003901170351106No Hit
TGCCATCTTGCCACATCTTAGCCTCCCGAGGGTATCCGCCAACTTGAGGA20.13003901170351106No Hit
TGCCATCTTGCCACATCTTGCCAAGGGTATCCGCCAACTTGAGGAGACCA20.13003901170351106No Hit
TCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT20.13003901170351106No Hit
TGCCATCTTGCCACATCTCCCAAGCAGTGGTATCAACGCAGAGTACATGG20.13003901170351106Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCCTCTCTGCCGTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA20.13003901170351106No Hit
TGCCGTCTAACCACATCTTGGGAGGGAAGGAAGGGTATCCGCCAACTTGA20.13003901170351106No Hit
TGCCCTCTCTGCCTTTTCTCAAGCAGTGGTATCAACGCAGAGTACATGGG20.13003901170351106Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT20.13003901170351106No Hit
GAGGACATCTTATGGTGCACTGAGGTATTGAGGAGACCAAGTCTCTGCTA20.13003901170351106No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC20.13003901170351106No Hit
GCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT20.13003901170351106No Hit
TGCCATCTTGCCACATCTTAGGGAGGGAAGGAAGGGTATCCGCCAACTTG20.13003901170351106No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGATATTTGGTTTCCCAGTCCACTA20.13003901170351106No Hit
TGCCATCTTGCCACATCTAGACGATGGGGAAGGGTATCCGCCAACTTGAG20.13003901170351106No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)