FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30976
Total Bases4.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT2054866.33522727272727No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC443614.320764462809917No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA14774.7682076446281No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT8112.618155991735537No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2190.7069989669421487No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG2180.7037706611570248No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG670.2162964876033058No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT530.17110020661157024No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT480.15495867768595042No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT470.15173037190082644No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT430.13881714876033058No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGCTTCCCAGT410.13236053719008264No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGC390.1259039256198347No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT390.1259039256198347No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT390.1259039256198347No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT390.1259039256198347No Hit
TGCCATCTTGCCACATCTACATTCTGTATCTGGAGGCTGTAGTCCCTTTT370.11944731404958676No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTCCCCAGT360.11621900826446281No Hit
GAGGACATCTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGTC360.11621900826446281No Hit
TGCCATCTTGCCACATCTTGCACCCCAATGCGGACCAGCCGCCGCAGCAG350.11299070247933883No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGCATTTGGTTTCCCAGT350.11299070247933883No Hit
GAGGACATCTTATGGTGCACTGGAATGGAAAGGGTATTTGGTTTCCCAGT340.10976239669421488No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTGTTTGGTTTCCCAGT330.1065340909090909No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGATATTTGGTTTCCCAGT320.10330578512396695No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)