FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30976
Total Bases4.4 Mbp
Sequences flagged as poor quality0
Sequence length35-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA1954863.10692148760331No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG480015.495867768595042No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC14354.632618801652892No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2210.7134555785123966No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCAACTGGTGTAGAACCATGTC2140.690857438016529No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1640.5294421487603306No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1390.44873450413223137No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1280.4132231404958678No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1200.38739669421487605No Hit
TACCTCAGTTTCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1080.34865702479338845No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1060.3422004132231405No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA970.3131456611570248No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG660.2130681818181818No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA620.20015495867768593No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG610.19692665289256198No Hit
GTTGGCGGATACCCTTCGAGAAATCTCGTCTCGACCAAGAGCTGAAGCTG570.1840134297520661No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG530.17110020661157024No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA500.16141528925619836No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA490.15818698347107438No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG470.15173037190082644No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATACTCCTTCA470.15173037190082644No Hit
TACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATACTCCTTCA460.1485020661157025No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACATGCAAAATACTCCTTCA450.1452737603305785No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG440.14204545454545456No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA430.13881714876033058No Hit
GTTGGCGGATACCCATGTCCCTTACACACACGCAAAATACTCCTTCAGCG410.13236053719008264No Hit
TACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATACTCCTTCA400.1291322314049587No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA380.12267561983471074No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAGTACTCCTTCA360.11621900826446281No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA340.10976239669421488No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA340.10976239669421488No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACGCGCAAAATACTCCTTCA330.1065340909090909No Hit
TACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATACTCCTTCA330.1065340909090909No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCG320.10330578512396695No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)