FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20611
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1168856.70758332929019No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC449321.799039347921013No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA8173.9639027703653387No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG4392.129930619572073No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG3311.605938576488283No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT3121.5137547911309495No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG1010.4900295958468779No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT670.325069137839018No Hit
GCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT390.18921934889136868No Hit
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC370.17951579253796518No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT370.17951579253796518No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT360.1746640143612634No Hit
TGCCATCTTGCCACATCTGGTACAGTTTCTTAGAAGCCGTTCAATAAGCA360.1746640143612634No Hit
GAGGACATCTTATGGTGCATGGGGGCAAACTGAGGTATTGAGGAGACCAA340.16496045800785988No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGCTTCCCAGT310.1504051234777546No Hit
TGCCATCTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT300.14555334530105282No Hit
GAGGACATCTTATGGTGTACTGAAATGGAAAGGGTATTTGGTTTCCCAGT290.14070156712435108No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT290.14070156712435108No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTCCCCAGT270.13099801077094755No Hit
TGCCATCTTGCCACATCTCTCCAGGGGCCTCCCTCCTGATCTGAGATCCT260.1261462325942458No Hit
TGCCATCTTGCCACATCTTGTACAGTTTCTTAGAAGCCGTTCAATAAGCA260.1261462325942458No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT250.12129445441754402No Hit
GAGGACATCTTATGGTGCACTGGAATGGAAAGGGTATTTGGTTTCCCAGT250.12129445441754402No Hit
TGCCATCTTGCCACATCTTATGGTTATGGCAGCAAGGGTATCCGCCAACT240.11644267624084227No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT220.10673911988743874No Hit
TGCCATCTTGCCACATCTTACCTTCCAGGTGGCAAGATAACCAAGCTTCG210.10188734171073699No Hit
GAGGACATCTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGTC210.10188734171073699No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)