FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3464
Total Bases501.8 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA72120.81408775981524No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA64418.59122401847575No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC38110.998845265588916No Hit
TGCCATCTTGCCACATCTTAGTCCTGTGTGACTTTCTCTACATTCACCTA1594.590069284064665No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1113.2043879907621244No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG1022.9445727482678983No Hit
TGCCATCTTGCCACATCTTACACACACCTCTAAATAACCCATTAGCTATC782.2517321016166285No Hit
TGCCATCTTGCCACATCTTGTGCCATCAAGGGTATCCGCCAACTTGAGGA732.107390300230947No Hit
TGCCATCTTGCCACATCTTAATCCTGTGTGACTTTCTCTACATTCACCTA411.1836027713625865No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACTTGAG371.0681293302540416No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT300.8660508083140877No Hit
TGCCATCTTGCCACATCTTAGACACACCTCTAAATAACCCATTAGCTATC280.8083140877598153No Hit
TGCCATCTTGCCACATCTACATTGTTCCAACATGCCAGAGGCTGTTCACC230.663972286374134No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG220.6351039260969976No Hit
TGCCATCTTGCCACATCTACTGTCCAACCACCCTGTCGAGTTTTCATCTG220.6351039260969976No Hit
TGCCATCTTGCCACATCTTACCAGCTGCTAATGCTGGCTCAGCAAGGGTA170.4907621247113164No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACTTGAGGAGACCAAGTCT160.4618937644341801No Hit
TGCCATCTTGCCACATCTTCCTCCCCAAGCAGTGGTATCAACGCAGAGTA160.4618937644341801Clontech Universal Primer Mix Long (96% over 25bp)
GAGGACATCTTATGGTGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC150.4330254041570438No Hit
TGCCATCTTGCCACATCTTAGGTGGCTACAGATGTGACTCCTTATCCTTG140.40415704387990764No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT120.3464203233256351No Hit
GAGGACATCTTGCCACATCTTAGTCCTGTGTGACTTTCTCTACATTCACC120.3464203233256351No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGTTGAGGAGACCAAGTCTCTGCT120.3464203233256351No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT100.28868360277136257No Hit
TGCCATCTTGCCACATCTTGTGCCATCAAGGGTATCCGCCAACACAGAAG100.28868360277136257No Hit
TGCCATCTTGCCACATCTCTCCTTCCCCACTTCCAGTTTATCCTCCAACC100.28868360277136257No Hit
TGCCATCTTGCCACATCTTAGAACACCTAGCCACTTAAGAAACCTAACGG100.28868360277136257No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTG90.25981524249422633No Hit
GAGGACATCTTATGGTGCACTGTGCATTCCTGTGCACACCATACCATAGA80.23094688221709006No Hit
TGCCATCTTGCCACATCTTCATCAACTTATCGACATTATTCCAAAGGGTA80.23094688221709006No Hit
TGCCATCTTGCCACATCTTGGTATCCGCCAACACAGAAGGGTATCCGCCA70.20207852193995382No Hit
TGCCGTCTAACCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC70.20207852193995382No Hit
GAGGACATCTTATGGTGCATGGGGGCAAACTGAGGTATTGAGGAGACCAA60.17321016166281755No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT60.17321016166281755No Hit
GAGGACATCTTGCCACATCTTGTGCCATCAAGGGTATCCGCCAACTTGAG60.17321016166281755No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT60.17321016166281755No Hit
GAGGACATCTTATGGTGCACTGAAGCAGTGGTATCAACGCAGAGTACATG60.17321016166281755Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCGTACCAGCTGCTAATGCTGGCTCAGCAAGGGTA60.17321016166281755No Hit
TGCCATCTTGCCACATCTTCGCCCAAGCAGTGGTATCAACGCAGAGTACA60.17321016166281755Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTAGCGAGCGCCGTTCCGAAGGGACGGGCGATG60.17321016166281755No Hit
TGCCATCTTGCCACATCTTATGCCATCAAGGGTATCCGCCAACTTGAGGA50.14434180138568128No Hit
TGCCATCTTGCCACATCTGGGTATCCGCCAACTTGAGGAGACCAAGTCTC50.14434180138568128No Hit
TGCCATCTTGCCACATCTACGTCCAAGGGTATCCGCCAACTTGAGGAGAC50.14434180138568128No Hit
TGCCATCTTGCCACATCTGGCTAATTTTTGTATTTTTAGTAGAGACGAGG50.14434180138568128No Hit
GAGGACATCTTATGGGGCAAACTGAGGTATTGAGGAGACCAAGTCTCTGC50.14434180138568128No Hit
TGCCATCTTGCCACATCTTGGTATCCGCCAACTTGAGGAGACCAAGTCTC40.11547344110854503No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACACAGA40.11547344110854503No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCGCTA40.11547344110854503No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT40.11547344110854503No Hit
TGCCATCTTGCCACATCTTAGGGTATCCGCCAACTTGAGGAGACCAAGTC40.11547344110854503No Hit
TGCCATCTTGCCACATCAGGGTATCCGCCAACTTGAGGAGACCAAGTCTC40.11547344110854503No Hit
TGCCATCTTGCCACATCTAGGGTATCCGCCAACTTGAGGAGACCAAGTCT40.11547344110854503No Hit
TGCCATCTTGCCACATCTTAGGCACACCTCTAAATAACCCATTAGCTATC40.11547344110854503No Hit
TGCCATCTTGCCACATCTTGACTGCCGGCGACGGCCGGGTATGGGCCCGA40.11547344110854503No Hit
AAGGTGGATGGGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT40.11547344110854503No Hit
TGCCATCTTCCACATCTTGTGCCATCAAGGGTATCCGCCAACTTGAGGAG40.11547344110854503No Hit
TGCCATCTTGCCACATCTGTGCAAGATACTCTGGGCCACAAATTCTTGCA40.11547344110854503No Hit
TGCCGTCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC40.11547344110854503No Hit
GAGGACATCTTATGGTGCACTGAGGTATTGAGGAGACCAAGTCTCTGCTA40.11547344110854503No Hit
GAGGACATCTTATGGTGCACTAAGCAGTGGTATCAACGCAGAGTACATGG40.11547344110854503Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTGTTTGTGAAGATGGAAGGGTATCCGCCAACTT40.11547344110854503No Hit
TGCCATCTTGCCACATCTTAGCTCTTGTTCGAGGGTATCCTTTCTGTCTG40.11547344110854503No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTACCGCCAACTTGAGG40.11547344110854503No Hit
TGCCATCTTGCCACATCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC40.11547344110854503No Hit
TGCCATCTTGCCACATCTTCCTCCCTGAAGCAGTGGTATCAACGCAGAGT40.11547344110854503Clontech Universal Primer Mix Long (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)